Human genomic DNA (HCC2157) (ATCC® CRL-2340D™)

Organism: Homo sapiens, human  / 

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Product Format frozen Approx.10 µg
Storage Conditions -20°C or colder in a manual defrost freezer. Avoid freeze-thawing.
Biosafety Level 1
Introduction

Cell Line Description:

This cell line was initiated on March 4, 1996 from a 48 year-old Black female, and took 8 months to establish.  The tumor was classified as TNM stage IIIA, grade 2, ductal carcinoma with 1 out of 9 lymph node metastasis.  The cells form ducts and morula-like structures (hollow spherical structures lined with a single layer of cells).  The cells are positive for expression of Her2-neu and for expression of p53. HCC2157 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19.  The cells are negative for expression of estrogen receptor (ER -), but positive for expression of progesterone receptor (PR +).  The DNA isolated from EBV-transformed lymphoblastoid cell line (HCC2157 BL) from the same patient is available as ATCC CRL-2341D.        

Quality Control Specifications

DNA Quality Control Information:

·         > 100 kb in size by agarose gel electrophoresis

·         No RNA detected in the agarose gel

·         A260/A280 ratio ³ 1.8

·         Tested and verified for PCR amplification and restriction digestion.
Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS