Cell Line Description:
This cell line was initiated on March 4, 1996 from a 48 year-old Black female, and took 8 months to establish. The tumor was classified as TNM stage IIIA, grade 2, ductal carcinoma with 1 out of 9 lymph node metastasis. The cells form ducts and morula-like structures (hollow spherical structures lined with a single layer of cells). The cells are positive for expression of Her2-neu and for expression of p53. HCC2157 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19. The cells are negative for expression of estrogen receptor (ER -), but positive for expression of progesterone receptor (PR +). The DNA isolated from EBV-transformed lymphoblastoid cell line (HCC2157 BL) from the same patient is available as ATCC CRL-2341D.
Quality Control Specifications
DNA Quality Control Information:
· > 100 kb in size by agarose gel electrophoresis
· No RNA detected in the agarose gel
· A260/A280 ratio ³ 1.8
· Tested and verified for PCR amplification and restriction digestion.