Human genomic DNA (HCC38) (ATCC® CRL-2314D)

Organism: Homo sapiens, human  / 

Permits and Restrictions

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Product Format frozen 10 µg
Storage Conditions -20°C or colder in a manual defrost freezer. Avoid freeze-thawing.
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Introduction

Cell Line Description:

This cell line was initiated on April 27, 1992 and took 32 months to establish. The cells are negative for expression of Her2-neu but positive for expression of p53. HCC38 was initiated from a 50-year-old white female with a prior history of leiomyosarcoma; her mother died of breast cancer. The tumor was classified as TMN Stage IIB, Grade 3, with 3/28 lymph node metastasis.  HCC38 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 [EGP2] and for cytokeratin 19, and is negative for expression of estrogen receptor (ER) and progesterone receptor (PR). HCC38 contains a homozygous deletion at 3p12. DNA from an EBV- transformed lymphoblastoid cell line, HCC38 BL, from the same patient is available as ATCC CRL-2346D.

Quality Control Specifications

DNA Quality Control Information:

·         > 100 kb in size by agarose gel electrophoresis

·         No RNA detected in the agarose gel

·         A260/A280 ratio ³ 1.8

·         Tested and verified for PCR amplification and restriction digestion.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation