Influenza A virus (H3N2) (ATCC® VR-1811)

Classification: Orthomyxoviridae, Influenzavirus A  /  Product Format: frozen

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Classification Orthomyxoviridae, Influenzavirus A
Agent Influenza A virus (H3N2)
Strain A/Virginia/ATCC6/2012
Biosafety Level 2
Product Format frozen
Storage Conditions -70°C or colder
Images ATCC VR-1811 images
Comments This item was isolated in cell culture by three passages in MDCK cells.
Effect on Host
CPE, rounding and sloughing
Recommended Host
MDCK cells (ATCC® CCL-34™)
Growth Conditions Temperature: 35°C
Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer three times with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 35°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-3 days at 35°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Effect on Host
CPE, rounding and sloughing
Source Clinical specimen from a patient in Virginia, 2012.
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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