Bovine rhinitis B virus (ATCC® VR-1806)

Classification: Picornaviridae, Aphthovirus  /  Product Format: frozen

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Classification Picornaviridae, Aphthovirus
Agent Bovine rhinitis B virus
Strain EC-11
Common Name Bovine rhinovirus 2
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions -70°C or colder
Images ATCC VR-1806 Cell Micrograph
Comments

Derived by adaptation from primary cell culture product ATCC® VR-392™ to MDBK cells (ATCC® CCL-22™). ATCC® VR-392™ and ATCC® VR-1806™ have not been compared for sequence or infectivity in primary cell culture and continuous tissue culture. Strain is 25-27 nm in diameter with characteristic rhinovirus structure. Antigenically distinct from Bovine rhinitis A virus, strain SD-1.

Effect on Host
CPE, cell rounding and sloughing
Recommended Host
MDBK cells (ATCC® CCL-22™)
Growth Conditions Temperature: 33°C
Recommendations for Infection: For best results, infection should be performed on a 70-80% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.1-0.5 recommended). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 33°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-7 days at 33°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Effect on Host
CPE, cell rounding and sloughing
Name of Depositor SB Mohanty, Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland
Source Lungs of a specific-pathogen-free Jersey calf
References

Betts AO, et al. Studies on a rhinovirus (EC11) derived from a calf. II. Disease in calves. J. Comp. Pathol. 81: 41-48, 1971. PubMed: 4326296

Reed SE, et al. Studies on a rhinovirus (EC11) derived from a calf. I. Isolation in calf tracheal organ cultures and characterization of the virus. J. Comp. Pathol. 81: 33-40, 1971. PubMed: 4326295

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customer Acceptance of Responsibility, ATCC Form 62 required for distribution.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Basic Documentation
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