Bovine rhinitis B virus (ATCC® VR-1806)

Classification: Picornaviridae, Aphthovirus  /  Product Format: frozen

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Classification Picornaviridae, Aphthovirus
Agent Bovine rhinitis B virus
Strain EC-11
Common Name Bovine rhinovirus 2
Biosafety Level 2
Product Format frozen
Storage Conditions -70°C or colder
Images ATCC VR-1806 Cell Micrograph
Comments

Derived by adaptation from primary cell culture product ATCC® VR-392™ to MDBK cells (ATCC® CCL-22™). ATCC® VR-392™ and ATCC® VR-1806™ have not been compared for sequence or infectivity in primary cell culture and continuous tissue culture. Strain is 25-27 nm in diameter with characteristic rhinovirus structure. Antigenically distinct from Bovine rhinitis A virus, strain SD-1.

Effect on Host
CPE, cell rounding and sloughing
Recommended Host
MDBK cells (ATCC® CCL-22™)
Growth Conditions Temperature: 33°C
Recommendations for Infection: For best results, infection should be performed on a 70-80% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.1-0.5 recommended). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 33°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-7 days at 33°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Effect on Host
CPE, cell rounding and sloughing
Name of Depositor SB Mohanty, Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland
Source Lungs of a specific-pathogen-free Jersey calf
References

Betts AO, et al. Studies on a rhinovirus (EC11) derived from a calf. II. Disease in calves. J. Comp. Pathol. 81: 41-48, 1971. PubMed: 4326296

Reed SE, et al. Studies on a rhinovirus (EC11) derived from a calf. I. Isolation in calf tracheal organ cultures and characterization of the virus. J. Comp. Pathol. 81: 33-40, 1971. PubMed: 4326295

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customer Acceptance of Responsibility, ATCC Form 62 required for distribution.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
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