Human rhinovirus 31 (ATCC® VR-1795)

Classification: Picornaviridae, Rhinovirus, Human rhinovirus A  /  Product Format: frozen

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Classification Picornaviridae, Rhinovirus, Human rhinovirus A
Agent Human rhinovirus 31
Strain 140 F
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions -70°C or colder
Images VR-1795 Images
Comments This item has been treated with diethyl ether to remove Mycoplasma orale at the ATCC.  The previous item number was VR-506™
Effect on Host
CPE, cell rounding and sloughing
Recommended Host
HeLa (ATCC® CCL-2™)
Growth Conditions Temperature: 33°C
Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.005 - 0.01 recommended). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 33°C in a humidified 5% CO2 atmosphere, while rocking. End adsorption by adding virus growth medium.
Incubation: 1-7 days at 33°C in a humidified 5% CO2 atmosphere while rocking, until CPE is progressed through 80% of the monolayer.
Effect on Host
CPE, cell rounding and sloughing
Name of Depositor VV Hamparian and RM Conant
Source Human throat washings
References

Conant RM, and Hamparian VV. Rhinoviruses: Basis for a Numbering System. I. HeLa cells for propagation and serologic procedures. J Immunol 100 (1): 107-113, 1968. PubMed: 4295059

Conant RM, and Hamparian VV. Rhinoviruses: Basis for a Numbering System. II. Serologic characterization of prototype strains. J Immunol 100 (1): 114-119, 1968. PubMed: 4295060

Connelly AP Jr., Hamre D. Virologic studies on acture respiratory disease in young adults. II. Characteristics and serolotic studies of three new Rhinoviruses. J. Lab. Clin. Med. 63: 30-43, 1964. PubMed: 14102901

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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