Human adenovirus 42 (ATCC® VR-1792)

Classification: Adenoviridae, Mastadenovirus, Human adenovirus D  /  Product Format: frozen 1 mL per vial

Classification Adenoviridae, Mastadenovirus, Human adenovirus D
Agent Human adenovirus 42
Strain 54/82
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Images VR-1792 Photomicrograph
Comments No cross-reactions by serum neutralization. Significant cross-reactions with Human adenovirus 15 and 22, bilaterally, by hemagglutinin inhibition assay. Hemagglutinates human or rhesus red blood cells. VR-1792™ was derived from VR-1304™ following treatment with diethyl ether to remove mycoplasma contamination.
Effect on Host
CPE, refractile rounding and sloughing
Recommended Host
HEp-2 (ATCC® CCL-23™)
Growth Conditions Temperature: 36°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 60-70% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.05). Adsorb 1-2 hours at 36°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-4 days
Effect on Host
CPE, refractile rounding and sloughing
Name of Depositor JC Hierholzer, Centers for Disease Control and Prevention; ATCC
Source Stool from an asymptomatic 1 year old male, Paris, 1982. Treated to remove mycoplasma at ATCC.
References

Hierholzer JC, et al. Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera. Arch. Virol. 121: 179-197, 1991. PubMed: 1759904

Ma Y, Mathews MB. Structure, function, and evolution of adenovirus-associated RNA: a phylogenetic approach. J. Virol. 70: 5083-5099, 1996. PubMed: 8764016

Wigand R, et al. A new human adenovirus of subgenus D: candidate adenovirus type 42. Arch. Virol. 94: 283-286, 1987. PubMed: 3034212