Human adenovirus 42 (ATCC® VR-1792)

Classification: Adenoviridae, Mastadenovirus, Human adenovirus D  /  Product Format: frozen 1 mL per vial

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Classification Adenoviridae, Mastadenovirus, Human adenovirus D
Agent Human adenovirus 42
Strain 54/82
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Images VR-1792 Photomicrograph
Comments No cross-reactions by serum neutralization. Significant cross-reactions with Human adenovirus 15 and 22, bilaterally, by hemagglutinin inhibition assay. Hemagglutinates human or rhesus red blood cells. VR-1792™ was derived from VR-1304™ following treatment with diethyl ether to remove mycoplasma contamination.
Effect on Host
CPE, refractile rounding and sloughing
Recommended Host
HEp-2 (ATCC® CCL-23™)
Growth Conditions Temperature: 36°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 60-70% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.05). Adsorb 1-2 hours at 36°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-4 days
Effect on Host
CPE, refractile rounding and sloughing
Name of Depositor JC Hierholzer, Centers for Disease Control and Prevention; ATCC
Source Stool from an asymptomatic 1 year old male, Paris, 1982. Treated to remove mycoplasma at ATCC.

Hierholzer JC, et al. Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera. Arch. Virol. 121: 179-197, 1991. PubMed: 1759904

Ma Y, Mathews MB. Structure, function, and evolution of adenovirus-associated RNA: a phylogenetic approach. J. Virol. 70: 5083-5099, 1996. PubMed: 8764016

Wigand R, et al. A new human adenovirus of subgenus D: candidate adenovirus type 42. Arch. Virol. 94: 283-286, 1987. PubMed: 3034212

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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