Encephalomyocarditis virus (ATCC® VR-1763)

Classification: Picornaviridae, Cardiovirus  /  Product Format: frozen 1 mL per vial

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Classification Picornaviridae, Cardiovirus
Agent Encephalomyocarditis virus
Strain MM
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Images VR-1763
Comments This item was deposited at ATCC® after six passages in weanling mice and three passages in suckling mice. A seed stock was produced by a single passage in BHK-21 cells.
Effect on Host CPE, refractile rounding and sloughing
Recommended Host BHK-21 (ATCC® CCL-10™)
Growth Conditions Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-95% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 15 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 1-3 days
Effect on Host CPE, refractile rounding and sloughing
Name of Depositor SE Grossberg, Medical College of Wisconsin
Source Isolated from dead mouse found in the basement of a patient with poliomyelitis, New York, 1942
References

Helwig FC, Schmidt EC. A filter-passing agent producing interstitial myocarditis in anthropoid apes and small animals. Science 102: 31-33, 1945.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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