Encephalomyocarditis virus (ATCC® VR-1763)

Classification: Picornaviridae, Cardiovirus  /  Product Format: frozen 1 mL per vial

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Classification Picornaviridae, Cardiovirus
Agent Encephalomyocarditis virus
Strain MM
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Images VR-1763
Comments This item was deposited at ATCC® after six passages in weanling mice and three passages in suckling mice. A seed stock was produced by a single passage in BHK-21 cells.
Effect on Host CPE, refractile rounding and sloughing
Recommended Host BHK-21 (ATCC® CCL-10™)
Growth Conditions Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-95% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 15 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 1-3 days
Effect on Host CPE, refractile rounding and sloughing
Name of Depositor SE Grossberg, Medical College of Wisconsin
Source Isolated from dead mouse found in the basement of a patient with poliomyelitis, New York, 1942

Helwig FC, Schmidt EC. A filter-passing agent producing interstitial myocarditis in anthropoid apes and small animals. Science 102: 31-33, 1945.

Oberste MS, et al. Human febrile illness caused by encephalomyocarditis virus infection, Peru. Emerg. Infect. Dis. 15: 640-646, 2009. PubMed: 19331761

Pindak FF, Schmidt JP. Propagation of MM virus in continuous cell lines. Appl. Microbiol. 17: 815-818, 1969. PubMed: 4307881

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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