Sandfly fever sicilian virus (ATCC® VR-1756)

Classification: Bunyaviridae, Phlebovirus  /  Product Format: frozen 1 mL per vial

Classification Bunyaviridae, Phlebovirus
Deposited As Phlebotomus virus (Sicily)
Agent Sandfly fever sicilian virus
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Effect on Host
CPE, rounding and sloughing
Recommended Host
BHK-21 [C-13] (ATCC® CCL-10™)
Growth Conditions Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 30-50% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 30 minutes at 37°C in a humidified 5% CO2 atmosphere, rocking every 10-15 minutes to redistribute inoculum. End adsorption by adding virus growth medium
Incubation: 4-5 days
Effect on Host
CPE, rounding and sloughing
Name of Depositor SE Grossberg, Medical College of Wisconsin
Chain of Custody ATCC <-- SE Grossberg, Medical College of Wisconsin