Bovine viral diarrhea virus 1 (ATCC® VR-1561)

Classification: Flaviviridae, Pestivirus  /  Product Format: frozen 1 mL per vial

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Classification Flaviviridae, Pestivirus
Agent Bovine viral diarrhea virus 1
Strain New York 1
Common Name Bovine viral diarrhea virus 1
Biosafety Level 2
Isolation
derived from existing strain
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder (or colder)
Comments Derived from same strain by passaging in the absence of bovine serum. Passage infected cells for optimal propagation of virus.
Effect on Host No CPE, detect by immunofluorescence or molecular-based procedures
Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Recommended Host BT (ATCC CRL-1390)
Growth Conditions
Temperature: 37°C

Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 60% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.

Incubation: 4-21 days
Cryopreservation
Storage temperature: -70°C
Effect on Host No CPE, detect by immunofluorescence or molecular-based procedures
Name of Depositor M Snyder, GA Erickson, The National Animal Disease Center
Special Collection ATCC
Source
Spleen from calf with diarrhea, New York, 1948
References

Baker JA. . Am. J. Vet. Res. 15: 525, 1954.

Kim SG, Dubovi EJ. A novel one-step single-tube RT-duplex PCR method with an internal control for detection of bovine viral diarrhea virus in bulk milk, blood, and follicular fluid samples. Biologicals. 31: 103-106, 2003. PubMed: 12770539

or colder

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Basic Documentation