Bovine viral diarrhea virus 1 (ATCC^® VR-1561^™)

Bovine viral diarrhea virus 1 ATCC^® VR-1561^™

frozen 1 mL per vial

For-Profit: $354.00 Non-Profit: $295.00

Qty:

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Classification	Flaviviridae, Pestivirus
Agent	Bovine viral diarrhea virus 1
Strain	New York 1
Common Name	Bovine viral diarrhea virus 1
Biosafety Level	2
Isolation	derived from existing strain
Product Format	frozen 1 mL per vial
Storage Conditions	-70°C or colder (or colder)

Comments	Derived from same strain by passaging in the absence of bovine serum. Passage infected cells for optimal propagation of virus.
Effect on Host	No CPE, detect by immunofluorescence or molecular-based procedures

Growth Medium	The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Recommended Host	BT (ATCC CRL-1390)
Growth Conditions	Temperature: 37°C Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 60% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm²) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO₂ atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 4-21 days
Cryopreservation	Storage temperature: -70°C

Effect on Host	No CPE, detect by immunofluorescence or molecular-based procedures

Name of Depositor	M Snyder, GA Erickson, The National Animal Disease Center
Special Collection	ATCC
Source	Spleen from calf with diarrhea, New York, 1948
References	Baker JA. . Am. J. Vet. Res. 15: 525, 1954. Kim SG, Dubovi EJ. A novel one-step single-tube RT-duplex PCR method with an internal control for detection of bovine viral diarrhea virus in bulk milk, blood, and follicular fluid samples. Biologicals. 31: 103-106, 2003. PubMed: 12770539 or colder

Permits

These permits may be required for shipping this product:

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.

Basic Documentation