Human parainfluenza virus 4b (ATCC® VR-1377)

Classification: Paramyxoviridae, Paramyxovirinae, Rubulavirus, Human parainfluenza virus 4  /  Product Format: frozen 1 mL per vial

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Classification Paramyxoviridae, Paramyxovirinae, Rubulavirus, Human parainfluenza virus 4
Deposited As Parainfluenza 4b
Agent Human parainfluenza virus 4b
Strain CH 19503
Applications
Respiratory research
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments Deposited as Parainfluenza 4b
Effect on Host
CPE, cell degeneration, clumping and sloughing
Recommended Host
NCI-H292 (ATCC® CRL-1848™)
Growth Conditions
Temperature: 37°C

Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere while rocking continuously.  End adsorption by adding virus growth medium.

Incubation: 6-11 days
Effect on Host
CPE, cell degeneration, clumping and sloughing
Name of Depositor JC Hierholzer, Centers for Disease Control and Prevention
Source Child with respiratory tract infection, Washington, DC, 1962
References

Canchola J, et al. Antigenic variation among newly isolated strains of Parainfluenza type 4 virus. Am. J. Hyg. 79: 357-364, 1964. PubMed: 14159955

Castells E, et al. NCI-H292 as an alternative cell line for the isolation and propagation of the human paramyxoviruses. Arch. Virol. 115: 277-288, 1990. PubMed: 22609024

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation