Hepatitis A virus (ATCC® VR-1357)

Classification: Picornaviridae, Hepatovirus  /  Product Format: frozen

Permits and Restrictions

View Permits

Classification Picornaviridae, Hepatovirus
Agent Hepatitis A virus
Strain PA21
Applications
Hepatitis A antigen can be detected in infected cells by IF and in infected cell culture supernate by ELISA. This item has fungal contamination that can, with difficulty, be controlled with Amphotericin B.
Enteric Research
Biosafety Level 2
Isolation
From liver tissue of a naturally infected owl monkey in Panama
Product Format frozen
Storage Conditions -70°C or colder (or colder)
Comments
PA21 is one of the better characterized primate HAV strains that differs significantly from the HM175-like isolates [Brown et al., J, Virol. 63: 4932-37, 1989]. Hepatitis A antigen can be detected in infected cells by IF and in infected cell culture supernate by ELISA. This item has fungal contamination that can, with difficulty, be controlled with Amphotericin B.
Effect on Host
Yes, in vivo effects: No CPE; Detect antigen by IF, ELISA or PCR
No CPE, detect by IF, ELISA or PCR
Growth Medium Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate supplemented with 2.5 ug/ml Amphotericin B Solution and 2% fetal bovine serum
Recommended Host

Recommended Host:  BSC-1 (ATCC CCL-26)

Growth Conditions
Atmosphere: 5% CO2 in air recommended
Temperature: 35°C
Incubation: 20 - 28 days; For best results cells should be 24-48 hours old and 70-80% confluent (not 100% confluent).
Cryopreservation
Storage temperature: -70°C
Effect on Host
Yes, in vivo effects: No CPE; Detect antigen by IF, ELISA or PCR
No CPE, detect by IF, ELISA or PCR
Name of Depositor LN Binn
Special Collection ATCC
Source
From liver tissue of a naturally infected owl monkey in Panama
References

Binn LN, et al. Primary isolation and serial passage of hepatitis A virus strains in primate cell cultures. J. Clin. Microbiol. 20: 28-33, 1984. PubMed: 6086708

or colder

low passage

or colder

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation