Leishmania major (Yakimoff and Schokhor) Bray et al. (ATCC® PRA-384)

Organism: Leishmania major (Yakimoff and Schokhor) Bray et al.  /  Depositor: R McMaster

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Deposited As Leishmania major NIH SD (MHOM/SN/74/SD)
Strain Designations MHOM/SN/74/SD
Application Wild type strain used in the targeted deletion of leishmanolysin (GP63) RefJoshi, PB, et al. Targeted gene deletion in Leishmania major identifies leishmanolysin (GP63) as a virulence factor. Mol. Biochem. Parasitol. 120: 33-40, 2002. PubMed: 11849703
Biosafety Level 2
Isolation Cutaneous leishmaniasis, Senegal, 1973
Product Format frozen
Storage Conditions Frozen: -70°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Protocols Section
Type Strain no
Comments The strain has also been referred in the scientific literature as NIH S strain (MHOM/SN/74/Seidman) RefNeva FA, et al. Cutaneous leishmaniasis--a case with persistent organisms after treatment in presence of normal immune response. Am. J. Trop. Med. Hyg. 28: 467-71, 1979. PubMed: 222157  RefMcDowell, MA, et al. Leishmania priming of human dendritic cells for CD40 ligand-induced interleukin-12p70 secretion is strain and species dependent. Infect. Immun. 70: 3994-4001, 2002. PubMed: 12117904 
Medium ATCC® Medium 2736: M199, Modified Medium
ATCC® Medium 1011: Diphasic blood agar medium
ATCC® Medium 807: Brain heart infusion blood agar
Growth Conditions
Temperature: 20°C to 25°C
Cryopreservation Harvest and Preservation
  1. Harvest cells from a culture which is at or near peak density by centrifugation at ~800 x g for 5 min.
  2. Adjust concentration of cells to 2 x 107/mL in fresh medium.  If the concentration is too low, centrifuge at ~800 x g for 5 minutes and resuspend the cell pellet with a volume of supernatant to yield the desired concentration.
  3. While cells are centrifuging, prepare a 10% (v/v) solution of sterile DMSO in fresh medium (broth).  The DMSO solution when first prepared will warm up due to chemical heat. The solution should be allowed to return to room temperature prior to use.
  4. Mix the cell preparation and the DMSO solution in equal portions. The final concentration will be 107 cells/mL and 5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should be no less than 15 min and no more than 30 min.
  5. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place the ampules in a Nalgene 1°C freezing apparatus.  Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.  (The cooling rate in this apparatus is approximately -1°C/min.)  If freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through heat of fusion.  At -40°C, plunge ampules into liquid nitrogen.
  7. Store in either the vapor or liquid phase of a nitrogen refrigerator.
  8. To thaw a frozen ampule, place it in a 35°C water bath such that the lip of the ampule remains above the water line. Thawing time is approximately 2 to 3 minutes.  Do not agitate the ampule.  Do not leave ampule in water bath after it is thawed.
  9. Remove the vial from the water bath immediately after thawing.  Aseptically transfer the contents of the ampule into 10 mL of fresh ATCC medium 2736. 
  10. Incubate the flask at 20-25°C with the cap screwed on tightly.
  11. Maintain as described above. 
Name of Depositor R McMaster
Chain of Custody ATCC <-- R McMaster <-- NIH
References

Neva FA, et al. Cutaneous leishmaniasis--a case with persistent organisms after treatment in presence of normal immune response. Am. J. Trop. Med. Hyg. 28: 467-71, 1979. PubMed: 222157

McDowell, MA, et al. Leishmania priming of human dendritic cells for CD40 ligand-induced interleukin-12p70 secretion is strain and species dependent. Infect. Immun. 70: 3994-4001, 2002. PubMed: 12117904

Joshi, PB, et al. Targeted gene deletion in Leishmania major identifies leishmanolysin (GP63) as a virulence factor. Mol. Biochem. Parasitol. 120: 33-40, 2002. PubMed: 11849703

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation