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Introduction
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Renal Epithelial Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC® Primary Cell Solutions system. This system is designed to support epithelial cells (e.g., renal proximal tubule epithelial cells) derived from normal human kidney. Renal Epithelial Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts.
To support the proliferation and plating efficiency of renal epithelial cells (e.g., Primary Renal Proximal Tubule Epithelial Cells, Normal, Human, ATCC® PCS-400-010; Primary Renal Cortical Epithelial Cells, Normal, Human, ATCC® PCS-400-011; Primary Renal Mixed Epithelial Cells, Normal, Human, ATCC® PCS-400-012), Renal Cell Basal Medium must be supplemented with the Renal Epithelial Cell Growth Kit (ATCC® PCS-400-040).
Using Renal Epithelial Cell Basal Medium supplemented with the Renal Epithelial Cell Growth Kit, the growth of renal epithelial cells is supported without the use of feeder layers, extracellular matrix proteins or other substrates.
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Quality Control Specifications
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Cell testing: Rate of proliferation and morphology.
pH: 7.5 ± 0.2
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Sterility Testing
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Negative for bacteria, fungi and yeast.
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