Cryptococcus sp. (ATCC® MYA-4950)

Strain Designations: HD-20  /  Product Format: frozen

Deposited As Cryptococcus sp.
Strain Designations HD-20
Application Produce pheomelanins
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain no
Preceptrol® no
Comments Produces pheomelanins
Epidemiological study
Morphology On YM media at 25°C after 8 days, colonies white to buff, mucoid, smooth, margin entire. Cells hyaline, globose to ellipsoidal, smooth, 2-5 X 2-4 µm.
Medium ATCC® Medium 28: Emmons' modification of Sabouraud's agar
ATCC® Medium 200: YM agar or YM broth
ATCC® Medium 1245: YEPD
Growth Conditions
Temperature: 20°C to 25°C
Atmosphere: Typical aerobic
Sequenced Data
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence:

AAGGATCATTAAAGATTGGCCGAGAGGCCTCATCTCTATATCCCTCAACCTCTGTGAACCGTGGACCCTCGGGTCTATTTTACAAACATCAGTGTAATGAACGTAATTATCATAACAAAAACAAAACTTTCAACAACGGATCTCTTGGCTCTCGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCTCTCGGTATTCCGAGAGGCATGCCTGTTTGAGTGTCATGAAATCTCAATCCCCTCGGGTTTTAGGACCCGGGTCGGACTTGGACATGGGCGTCTGCCGGTTAAACGGCTCGCCTCAAATGACTTAGTGGATCTCTCAGCATCCGTGACAGACGTAATAAGTTTCGTCTTGTCCCTTGCAGATGAGTCCGCTCACAACCTGCCATCGCGCACTTTTAGACTCTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA


D1D2 region of the 28S ribosomal RNA gene:

ATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACGGCGAGTGAACCGGGAAGAGCTCAAATTTGAAATCTGGCGTCCTCAGGGCGTCCGAGTTGTAATCTATAGAGGCGTTTTCCGTGCCGGACCGTGTCCAAGTCCCTTGGAACAGGGTATCAAAGAGGGTGACAATCCCGTACTTGACACGACTACCGGTGCTCTGTGATACGTCTTCTACGAGTCGAGTTGTTTGGGAATGCAGCTCAAAATGGGTGGTGAGTTCCATCTAAAGCTAAATATTGGCGAGAGACCGATAGCGAACAAGTACCGTGAGGGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAACAGTACGTGAAATTGTTGAAAGGGAAACGATTGAAGTCAGTCGTGACCGAGAGGCTCAGCCGGCTCTGCCGGTGTATTCCCCTCGGTCGGGTCAACATCAGTTTTGTCCGGTGGATAAGGGCGGCAGGAATGTGGCACCCTCGGGTGTGTTATAGCCTGCTGTCGCATACACCGGGTGAGACTGAGGAACGCAGCTCGCCTTTATGGCCGGGGCCCGCCCACGTACGAGCTTAGGATGTTGACGTAATGGCTTTAAAC

Morphology On YM media at 25°C after 8 days, colonies white to buff, mucoid, smooth, margin entire. Cells hyaline, globose to ellipsoidal, smooth, 2-5 X 2-4 µm.
Name of Depositor H Dhillon
Chain of Custody ATCC <-- H Dhillon
Isolation Juglan regia, Solan, Himachal Pradesh, India.
References

Dhillon H, Sharma DR. Extracellular Deoxyribonuclease and Hemolysins Production by Cryptococcus species. Int. J. New Horiz. Pharm. Biomed. Sci. 1: 13-24, 2013.