pRS327 (ATCC® MBA-14)

Applications: cloning vector  /  Depositors: DJ Stillman

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Designations pRS327
Depositors DJ Stillman
Biosafety Level 1
Vector Information
Size (kb): 9.56
Vector: pRS327 (plasmid)
Type: shuttle
Construction: pUC
Marker(s):AmpR, LYS2
Features: eukaryotic selectable marker cassette: LYS2
insert detection: LacZ
Applications
cloning vector
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Sikorski RS, Boeke JD. In vitro mutagenesis and plasmid shuffling: from cloned gene to mutant gene. Methods Enzymol. 194: 302-318, 1991. PubMed: 2005795

Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436

Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801

Eriksson P, et al., pRS yeast vectors with a LYS2 marker. Biotechniques 36: 212-213, 2004.

Shipped FZ
Shipping Information

Shipped: Freeze dried Escherichia coli DH5 alpha containing the plasmid.

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Product Sheet
Product Sheet
Product Sheet