B6H12.2 (ATCC® HB-9771™)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte

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These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with vitronectin receptors on human polymorphonuclear leukocytes (PMN, neutrophils) and blocks the receptor binding to the tripeptide Arg-Gly-Asp (RGD).
Derivation
Animals were immunized with purified integrin associated protein (which is physically and functionally associated with vitronectin receptors).
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against integrin associated protein (CD47) and vitonectin receptor (VnR)
Comments
Animals were immunized with purified integrin associated protein (which is physically and functionally associated with vitronectin receptors).
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with vitronectin receptors on human polymorphonuclear leukocytes (PMN, neutrophils) and blocks the receptor binding to the tripeptide Arg-Gly-Asp (RGD).
The antibody also inhibits RGD stimulated phagocytosis.
It precipitates a heterodimer of apparent molecular weights 140000 and 105000 daltons.
The antibody does not react with gpIIb/III or the LFA-1/Mac-1 family of cellular adhesion receptors.
Complete Growth Medium Iscove's modified Dulbecco's medium, 80%; fetal bovine serum, 20%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG1
Name of Depositor Washington Univ.
References

Brown EJ. Novel monoclonal antibodies. US Patent 5,057,604 dated Oct 15 1991

Lindberg FP, et al. Rh-related antigen CD47 is the signal-transducer integrin-associated protein. J. Biol. Chem. 269: 1567-1570, 1994. PubMed: 8294396

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
References

Brown EJ. Novel monoclonal antibodies. US Patent 5,057,604 dated Oct 15 1991

Lindberg FP, et al. Rh-related antigen CD47 is the signal-transducer integrin-associated protein. J. Biol. Chem. 269: 1567-1570, 1994. PubMed: 8294396