H21F8-1 (ATCC® CRL-8018™) Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) / Cell Type: hybridoma: B lymphocyte General Information Characteristics Culture Method Specifications History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) Cell Type hybridoma: B lymphocyte Product Format frozen Morphology lymphoblast Culture Properties suspension Biosafety Level 1 Disease hepatitis Derivation Spleen cells were fused with P3-NSI/1Ag4-1 myeloma cells. Genes Expressed immunoglobulin; monoclonal antibody; against hepatitis B virus surface antigen (HBsAg) Comments Animals were immunized with hepatitis B virus surface antigen.Spleen cells were fused with P3-NSI/1Ag4-1 myeloma cells. Complete Growth Medium Iscove's modified Dulbecco's medium, 80%; fetal bovine serum, 20% Subculturing Medium Renewal: Every 2 to 3 daysCultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. Isotype IgM Name of Depositor Massachusetts General Hospital References Wands JR, Zurawski VR Jr.. Process for producing antibodies to hepatitis virus and cell lines therefor. US Patent 4,271,145 dated Jun 2 1981 Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis MSDS References Wands JR, Zurawski VR Jr.. Process for producing antibodies to hepatitis virus and cell lines therefor. US Patent 4,271,145 dated Jun 2 1981