H21F8-1 (ATCC® CRL-8018)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  /  Disease: hepatitis

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease hepatitis
Derivation
Spleen cells were fused with P3-NSI/1Ag4-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against hepatitis B virus surface antigen (HBsAg)
Cellular Products
immunoglobulin; monoclonal antibody; against hepatitis B virus surface antigen (HBsAg)
Comments
Animals were immunized with hepatitis B virus surface antigen.
Spleen cells were fused with P3-NSI/1Ag4-1 myeloma cells.
Complete Growth Medium Iscove's modified Dulbecco's medium, 80%; fetal bovine serum, 20%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgM
Name of Depositor Massachusetts General Hospital
References

Wands JR, Zurawski VR Jr.. Process for producing antibodies to hepatitis virus and cell lines therefor. US Patent 4,271,145 dated Jun 2 1981

Basic Documentation
References

Wands JR, Zurawski VR Jr.. Process for producing antibodies to hepatitis virus and cell lines therefor. US Patent 4,271,145 dated Jun 2 1981