SU-DHL-8 (ATCC® CRL-2961™) Organism: Homo sapiens, human / Tissue: peritoneal effusion / Cell Type: B lymphocyte General Information Characteristics Culture Method Specifications History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Organism Homo sapiens, human Tissue peritoneal effusion Cell Type B lymphocyte Product Format frozen Morphology lymphoblast-like Culture Properties suspension, grow as single cells and small clusters Biosafety Level 1 Disease Large cell lymphoma Age 59 years Gender Male Ethnicity Caucasian Storage Conditions liquid nitrogen vapor phase Clinical Data 59 years Caucasian Male Receptor Expression Surface Receptors: Fc (not expressed), IgMEAC (not expressed), IgGEA (not expressed), E (not expressed) Genes Expressed Ig +; IgM-, IgG-, IgA-, IgD-, Lambda-, Kappa- Comments The depositor states that this cell line is negative for Epstein-Barr Virus (EBV). ATCC confirmed this cell line is negative for the presence of Epstein-Barr viral DNA sequences via PCR. This cell line is negative for Canadida ingestion. This is a null cell line (absence of all known markers) based on the classification of diffuse histiocytic lymphomas. These cells exhibit no response to LY294002-induced apoptosis. Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Subculturing Protocol: Cultures can be maintained by the addition of fresh medium. Subculture the cells when cell density is ~ 1.0 x 106 to 2.5 x 106 cells/mL for higher viability. An inoculum of 1.0 x 105 to 4.0 x 105 cells/mL is recommended. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:10 is recommended. Medium renewal: Every 2 to 4 days (depending on cell density) Culture Conditions Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% STR Profile D5S818: 11, 13 D13S317: 11, 13 D7S820: 8 D16S539: 12 CSF1PO: 11, 12 vWA: 15, 19 THO1: 6, 9 TPOX: 8 Amelogenin: X Year of Origin 1978 References Epstein AL, et al. Biology of the human malignant lymphomas. IV. Functional characterization of ten diffuse histiocytic lymphoma cell lines. Cancer. 42(5): 2379-2391, 1978. PubMed: 214220 Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis MSDS References Epstein AL, et al. Biology of the human malignant lymphomas. IV. Functional characterization of ten diffuse histiocytic lymphoma cell lines. Cancer. 42(5): 2379-2391, 1978. PubMed: 214220