BCL2 Jurkat (ATCC® CRL-2899)

Organism: Homo sapiens, human  /  Cell Type: T-Cell/ T Lymphocyte  /  Tissue: peripheral blood  /  Disease: Acute T-Cell Leukemia

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Organism Homo sapiens, human
Tissue peripheral blood
Cell Type T-Cell/ T Lymphocyte
Product Format frozen
Morphology Lymphoid-like
Culture Properties suspension
Biosafety Level 2
Disease Acute T-Cell Leukemia
Age 14 years
Gender Male
Applications

This cell line over-expresses BCL-2, and is useful to study cell apoptosis, BCL-2 function and its pathway.

Storage Conditions liquid nitrogen vapor phase
Images CRL-2899 Cell Micrograph
Derivation

The BCL2 Jurkat cell line was derived by transfecting human Jurkat T cells with the pSFFV-neo mammalian expression vector containing the human BCL-2 ORF insert and a neomycin-resistant gene (Addgene # 3349).  Stable neomycin (Neo)-resistant single-cell-originated clones were isolated and expanded by selection in medium containing 1 mg/mL G418 for 14 days (1).

Tumorigenic Yes
Comments

The BCL2 Jurkat cell line was derived by transfecting human Jurkat T cells with the pSFFV-neo mammalian expression vector containing the human BCL-2 ORF insert and a neomycin-resistant gene (Addgene # 3349).  

Stable neomycin (Neo)-resistant single-cell-originated clones were isolated and expanded by selection in medium containing 1 mg/mL G418 for 14 days (1).  The BCL2 Jurkat cell line produces over-expression of human BCL-2.

Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: 10% FBS and 200 mcg/mL G418.
Subculturing

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium. An inoculum of 2 x 105 to 3 x 105 cells/mL is recommended. Subculture when cell concentration is 2 x 106 cells/mL.

Subcultivation ratio: A subcultivation ratio of 1:2 to 1:10 is recommended.
Medium renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Culture Conditions Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
D5S818: 9
D13S317: 8, 12
D7S820: 8, 12
D16S539: 11
vWA: 18, 19
TH01: 6, 9.3
Amelogenin: X Y
TPOX: 8, 10
CSF1PO: 11, 12
Name of Depositor Stanley Korsmeyer
Year of Origin 2003
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation