A7 [M2A7] (ATCC® CRL-2500™)

Organism: Homo sapiens, human  /  Cell Type: Melanoma

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism Homo sapiens, human
Cell Type Melanoma
Product Format frozen
Morphology melanocytic
Culture Properties adherent
Biosafety Level 1
Applications
M2 cells are severely deficient in filamin-1 mRNA and protein.
The filament-1 gene on the X chromosome is present in these cells.
A7 cells are useful as a control for studying these systems.
Storage Conditions liquid nitrogen vapor phase
Genes Expressed
filamin-1 (actin-binding protein ABP or ABP-280)
Comments
M2 cells are severely deficient in filamin-1 mRNA and protein. Filamin-1, also known as actin-binding protein (ABP, APB-280), cross-links actin filaments in vitro and connects these filaments to plasma membrane glycoproteins.
The filament-1 gene on the X chromosome is present in these cells.
ABP (filamin-1) was transfected into M2 cells in a mammalian expression vector (LK444) containing full length human ABP recombinant DNA (LK-APB).
A7 cells express levels of filamin-1 comparable to wild-type filamin-1 containing melanoma cells.
Filamin-1 is essential for cell migration and has been shown to bind partners in cell signal transduction, cell membrane sorting and cytoskeleton-membrane association.
A7 cells are useful as a control for studying these systems.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium:
  • 10 mM HEPES
  • 0.5 mg/ml G418
  • fetal bovine serum to a final concentration of 10%
Subculturing
Protocol: Remove medium, and rinse with 0.25% trypsin - 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: culture medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Name of Depositor TP Stossel
References

Cunningham CC, et al. Actin-binding protein requirement for cortical stability and efficient locomotion. Science 255: 325-327, 1992. PubMed: 1549777

Cunningham CC. Actin polymerization and intracellular solvent flow in cell surface blebbing. J. Cell Biol. 129: 1589-1599, 1995. PubMed: 7790356

Cantiello HF, et al. Actin-binding protein contributes to cell volume regulatory ion channel activation in melanoma cells. J. Biol. Chem. 268: 4596-4599, 1993. PubMed: 8383118

Byers HR, et al. Cell migration and actin organization in cultured human primary, recurrent cutaneous and metastatic melanoma. Time-lapse and image analysis. Am. J. Pathol. 139: 423-435, 1991. PubMed: 1867326

A7 is a stably transfected cell line derived from the M2 (RPM-MC) cell line. M2 was established from the tumor of a patient with malignant melanoma.

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
References

Cunningham CC, et al. Actin-binding protein requirement for cortical stability and efficient locomotion. Science 255: 325-327, 1992. PubMed: 1549777

Cunningham CC. Actin polymerization and intracellular solvent flow in cell surface blebbing. J. Cell Biol. 129: 1589-1599, 1995. PubMed: 7790356

Cantiello HF, et al. Actin-binding protein contributes to cell volume regulatory ion channel activation in melanoma cells. J. Biol. Chem. 268: 4596-4599, 1993. PubMed: 8383118

Byers HR, et al. Cell migration and actin organization in cultured human primary, recurrent cutaneous and metastatic melanoma. Time-lapse and image analysis. Am. J. Pathol. 139: 423-435, 1991. PubMed: 1867326

A7 is a stably transfected cell line derived from the M2 (RPM-MC) cell line. M2 was established from the tumor of a patient with malignant melanoma.