HPAC (ATCC® CRL-2119™)

Organism: Homo sapiens, human  /  Tissue: pancreas  /  Cell Type: Epithelial

Organism Homo sapiens, human
Tissue
pancreas
Cell Type Epithelial
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease adenocarcinoma
Age 64 years
Gender female
Ethnicity Caucasian
Applications
Immunohistochemical staining revealed that HPAC cells express the pancreatic ductal epithelium marker DU-PAN-2 as well as antigens recognized by the monoclonal antibodies HMFG1 and AUA1.
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 61
Derivation
HPAC is a pancreatic adenocarcinoma epithelial cell line derived in 1985 from a nude mouse xenograft of a primary tumor removed from the head of the pancreas of a woman with moderate to well differentiated pancreatic adenocarcinoma of ductal origin.
Clinical Data
HPAC is a pancreatic adenocarcinoma epithelial cell line derived in 1985 from a nude mouse xenograft of a primary tumor removed from the head of the pancreas of a woman with moderate to well differentiated pancreatic adenocarcinoma of ductal origin.
Receptor Expression
epidermal growth factor (EGF), expressed
glucocorticoid, expressed
epidermal growth factor (EGF); glucocorticoid
Genes Expressed
HPAC cells are positive for keratin and negative for vimentin and chromogranin A.
Tumorigenic Yes
Effects
Yes, the cells form tumors in athymic nude mice at the site of inoculation which are histologically similar to the tumor of origin
Yes, the cells have a colony forming efficiency of 64% on plastic and 3.2% in agarose.
Comments
HPAC proliferation is stimulated by insulin, insulin-like growth factor I (IGF-I), epidermal growth factor (EGF), and transforming growth factor alpha (TGF alpha).
Cell growth is suppressed by dexamethasone and other glucocorticoids.
Crude HPAC cell exacts contained significant concentrations of tumor-associated antigens CEA, CA 125, and CA 19-9.
In culture, HPAC cells form monolayers of morphologically heterogenous polar epithelial cells.
HPAC is the first reported human pancreatic adenocarcinoma cell line to express a functional glucocorticoid receptor.
Complete Growth Medium A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES and 0.5 mM sodium pyruvate supplemented with 0.002 mg/ml insulin, 0.005 mg/ml transferrin, 40 ng/ml hydrocortisone, 10 ng/ml epidermal growth factor and 5% fetal bovine serum
Subculturing
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 3 to 4 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Temperature: 37.0°C
STR Profile
Amelogenin: X
CSF1PO: 13
D13S317: 11
D16S539: 9,10
D5S818: 12
D7S820: 10,12
THO1: 9.3
TPOX: 10,11
vWA: 15,17
Population Doubling Time 41 hrs
Name of Depositor WR Gower
Year of Origin 1985
References

Gower WR Jr., et al. HPAC, a new human glucocorticoid-sensitive pancreatic ductal adenocarcinoma cell line. In Vitro Cell. Dev. Biol. 30A: 151-161, 1994.

Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
References

Gower WR Jr., et al. HPAC, a new human glucocorticoid-sensitive pancreatic ductal adenocarcinoma cell line. In Vitro Cell. Dev. Biol. 30A: 151-161, 1994.