LLC-PK1 (ATCC® CL-101)

Organism: Sus scrofa, pig  /  Tissue: kidney  / 

Organism Sus scrofa, pig
Tissue kidney
Product Format frozen
Morphology epithelial
Biosafety Level 1
Age 3 to 4 weeks
Gender male
Strain Hampshire
Storage Conditions liquid nitrogen vapor phase
Clinical Data
male
Genes Expressed
plasminogen activator
Cellular Products
plasminogen activator
Complete Growth Medium The base medium for this cell line is Medium 199 containing 1.5 g/L to 2.2 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 3%.
Subculturing
Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.>
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
  4. Add 2.0 to 3.0 mL of complete growth medium and aspirate cells by gently pipetting
  5. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: Twice per week
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Name of Depositor Eli Lilly & Co.
References

Hull RN, Huseby RM. Enhanced production of plasminogen activator. US Patent 3,904,480 dated Sep 9 1975

Perantoni A, Berman JJ. Properties of Wilms' tumor line (TuWi) and pig kidney line (LLC-PK1) typical of normal kidney tubular epithelium. In Vitro 15: 446-454, 1979. PubMed: 225262

Loffler S, et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321

Basic Documentation
References

Hull RN, Huseby RM. Enhanced production of plasminogen activator. US Patent 3,904,480 dated Sep 9 1975

Perantoni A, Berman JJ. Properties of Wilms' tumor line (TuWi) and pig kidney line (LLC-PK1) typical of normal kidney tubular epithelium. In Vitro 15: 446-454, 1979. PubMed: 225262

Loffler S, et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321