K-BALB (K-234) (ATCC® CCL-163.3)

Organism: Mus musculus, mouse  /  Cell Type: fibroblastKirsten murine sarcoma virus (Ki-MSV) transformed  / 

Organism Mus musculus, mouse
Cell Type fibroblastKirsten murine sarcoma virus (Ki-MSV) transformed
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 14 to 17 days gestation embryo
Strain BALB/c
Applications
They do not produce Ki-MSV.
Antigen Expression
H-2d
Genes Expressed
H-2d
Tumorigenic Yes
Effects
Yes, in BALB/c mice
Comments
They do not produce Ki-MSV.
Complete Growth Medium Dulbecco's modified Eagle's medium, 90%; bovine calf serum, 10%
Subculturing
Protocol: Remove medium, add fresh 0.1% trypsin for 3 to 5 minutes, remove trypsin and let the culture sit at room temperature for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 5 to 7 days.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: Twice per week
Culture Conditions
Temperature: 37.0°C
Name of Depositor S Aaronson
References

Aaronson SA, Weaver CA. Characterization of murine sarcoma virus (Kirsten) transformation of mouse and human cells. J. Gen. Virol. 13: 245-252, 1971. PubMed: 4333712

Stephenson JR, Aaronson SA. Antigenic properties of murine sarcoma virus-transformed BALB-3T3 nonproducer cells. J. Exp. Med. 135: 503-515, 1972. PubMed: 4550769

Basic Documentation
References

Aaronson SA, Weaver CA. Characterization of murine sarcoma virus (Kirsten) transformation of mouse and human cells. J. Gen. Virol. 13: 245-252, 1971. PubMed: 4333712

Stephenson JR, Aaronson SA. Antigenic properties of murine sarcoma virus-transformed BALB-3T3 nonproducer cells. J. Exp. Med. 135: 503-515, 1972. PubMed: 4550769