|
Vector Information
|
Size (kb): 5.4159998893737790 DESCRIPTION OF VECTOR:
Intact vector size: 5.416
Type of vector: plasmid
Cloning sites: XhoI SalI ClaI EcoRI PstI SmaI BamHI SpeI
Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI
Other unique sites: SacI
Construction: pRS414
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): TRP1, ->, 200-1213
terminator: CYC1, ->, 1895-2155
MCS: SalI...XbaI, ->, 2161-2219
promoter for expression: TEF, <-, 2219-2621
marker(s): ampR, <-, 3868-4798
replicon: CEN6/ARSH4, ->, 4902-5416 Vector: p414 TEF (plasmid) Promoters: Promoter for expression TEF Construction: pRS414 Marker(s):TRP1,ampR Construct size (kb): 5.415999889373779 Features: marker(s): TRP1
marker(s): ampR
promoter for expression: TEF
replicon: CEN6/ARSH4
MCS: SalI...XbaI
terminator: CYC1
|
|
Applications
|
YX-type (expression) shuttle vector vector with low copy number
|
|
Comments
|
Restriction digests of the clone give the following sizes (kb): BamHI--5.6; EcoRI--5.6; SacI/XbaI--3.1, 1.7, 0.42. Low copy number shuttle expression vector.
|
|
References
|
Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504
|
|
Shipping Information
|
Distributed: freeze-dried
|