lambdaACT2 - human breast cDNA library (ATCC® 87295)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: SJ Elledge

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Designations lambdaACT2 - human breast cDNA library
Species Homo sapiens, human
Depositors SJ Elledge
Genome: human
Tissue: breast
Type of DNA: cDNA
Insert size: 0.7 – 7 kb
Number of independent recombinants: 1.0 x 108
Non-recombinants (%): 5.0
Insert 5’ end:
Insert 3’ end: XhoI adapters
Titer: 9.5 x 108 pfu/mL

Vector: lambdaACTII
Intact vector size (kb): 42.300
Type of vector: phage
    Cloning sites:   NcoI SfiI SmaI BamHI EcoRI XhoI BglII
    Promoter: ADCI
    Replicon: pMB1, 2 micron, lambda
    Repressor gene: cI857
    Terminator: ADC1
Construction: lambdaYES, pACT2 (ATCC#87006)
Vector ends: EcoRI, XhoI
Markers: ampR, LEU2
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

In yeast, the ADH promoter drives expression of a fusion protein consisting of the GAL4 activation domain, a hemagglutinin tag, and the sequences directed by the insert.

Durfee T, et al. The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit. Genes Dev. 7: 555-569, 1993. PubMed: 8384581

Stephen J Elledge, personal communication

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