lambdaACT2 - human breast cDNA library (ATCC® 87295)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: SJ Elledge

Designations lambdaACT2 - human breast cDNA library
Species Homo sapiens, human
Depositors SJ Elledge
Origin
breast
Vector
Genome: human
Tissue: breast
Type of DNA: cDNA
Insert size: 0.7 – 7 kb
Number of independent recombinants: 1.0 x 108
Non-recombinants (%): 5.0
Insert 5’ end:
Insert 3’ end: XhoI adapters
Titer: 9.5 x 108 pfu/mL

Vector: lambdaACTII
Intact vector size (kb): 42.300
Type of vector: phage
Features:
    Cloning sites:   NcoI SfiI SmaI BamHI EcoRI XhoI BglII
    Promoter: ADCI
    Replicon: pMB1, 2 micron, lambda
    Repressor gene: cI857
    Terminator: ADC1
Construction: lambdaYES, pACT2 (ATCC#87006)
Vector ends: EcoRI, XhoI
Markers: ampR, LEU2
Biosafety Level 1
Comments
In yeast, the ADH promoter drives expression of a fusion protein consisting of the GAL4 activation domain, a hemagglutinin tag, and the sequences directed by the insert.
References

Durfee T, et al. The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit. Genes Dev. 7: 555-569, 1993. PubMed: 8384581

Stephen J Elledge, personal communication

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