pBSL193 (ATCC® 87152)

Applications: contains easily purifiable cassette(s) for construction omega-tet  /  Depositors: MF Alexeyev

Designations pBSL193
Depositors MF Alexeyev
Biosafety Level 1
Vector Information
Size (kb): 5.2750000953674320
Vector: pBSL193 (phagemid)
Promoters: Promoter for in vitro transcription T7 (phi10)
Construction: pBluescript
Marker(s):ampR,tetR
Construct size (kb): 5.275000095367432
Features: marker(s): ampR
promoter for in vitro transcription: T7 (phi10)
replicon: f1
replicon: pMB1
MCS: KpnI...SacI MluI
MCS: MluI KpnI...SacI
coding sequence: tetR
Applications
contains easily purifiable cassette(s) for construction omega-tet
Comments
Restriction digests of the clone give the following sizes (kb):BamHI--3.0, 2.3; EcoRI--3.0, 1.45, 0.6, 0.4; KpnI--3.0, 2.3; MluI--3.15, 2.2; SstI--3.0, 2.35.
Plasmid useful for generation of marked deletions and insertions in cloned DNA in vitro. Contains an omega - Tc element flanked by multiple cloning sites duplicated in tandem orientation.
Corresponds to the EcoRI/StyI fragment of pBR322, with BamHI, SalI, AccI, HincII, and HindIII sites removed.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718

Shipped freeze-dried
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