Oxytricha trifallax (ATCC® 50532)

Strain Designations: SLC 89  /  Depositor: GA Herrick  /  Biosafety Level: 1

Permits and Restrictions

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Strain Designations SLC 89
Biosafety Level 1
Isolation Unknown
Product Format frozen
Storage Conditions Frozen: -70°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Protocols Section
Type Strain no
Medium Medium 802: Sonneborn's Paramecium medium
Growth Conditions Temperature: 20°C to 25°C
Culture system: Xenic, with Enterobacter aerogenes ATCC 13048 and unidentified microalgae as food sources
Cryopreservation Reagents
Cryoprotective Solution
DMSO, 2.0 mL 
Fresh growth medium w/o bacteria, 8.0 mL

Harvest and Preservation
  1. Mix the components in the order listed. When the medium is added to the DMSO the solution will warm up due to chemical heat. 
  2. Harvest cells from a culture that is at or near peak density by centrifugation at 125 x g for 5 min. 
  3. Adjust the concentration of cells at least 1 x 105/mL in fresh medium. 
  4. Mix the cell preparation and the cryoprotective solution in equal portions. 
  5. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. 
  7. Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator. 
  8. To establish a culture from the frozen state add 1.0 mL bacterized ATCC medium 802 to the frozen ampule and place it in a 35°C water bath.  Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial. 
  9. Immediately after thawing, do not leave in water bath, aseptically remove the contents of the ampule and inoculate onto the surface of an ATCC medium 919 (non-nutrient agar) plate containing an overlay of 15.0 mL of bacterized ATCC medium 802. 
  10. Incubate at 25°C with the cap on loosely. 
  11. Once the culture is established, transfer 0.5 mL to 5.0 mL of bacterized ATCC medium 802. 
  12. Follow the protocol for maintenance of culture.
Name of Depositor GA Herrick
Geographical Isolation unknown
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation