pMMB207 (ATCC® 37809)

Applications: shuttle vector  /  Depositors: VM Morales

Permits and Restrictions

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Designations pMMB207
Depositors VM Morales
Biosafety Level 1
Vector Information
Size (kb): 9.0719995498657230
Vector: pMMB207 (plasmid)
Promoters: Promoter tac
Construction: pMMB206, pMMB67EH
Marker(s):cmlR
Construct size (kb): 9.071999549865723
Features: marker(s): cmlR
promoter: tac
replicon: oriV, oriT (IncQ)
transcription terminator: rrnB
Applications
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--9.1; EcoRV--5.3, 3.9; SspI--4.1, 1.8, 1.6, 1.0, 0.7; EcoRI/MluI--8.3, 0.8.
Does not have a start codon at a suitable distance after the ribosome-binding site, so translation will be efficient only if the insert contains an RBS followed appropriately by a start codon.
pMMB207 (ATCC 37809) and pMMB208 (ATCC 37810) differ in the orientation of the multiple cloning site with pMMB207 having the EcoRI site near the promoter and pMMB208 having the HindIII site nearer.
Constructed by replacing the MluI/XmnI fragment of pMMB206 (taclacUV5 promoter, M13mp8 polylinker, lacZalpha) with the MluI/XmnI fragment of pMMB67EH (tac promoter, M13mp18 polylinker without lacZalpha).
Media Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol
Growth Conditions
Temperature: 37.0°C
References

Morales VM, et al. A series of wide-host-range low-copy-number vectors that allow direct screening for recombinants. Gene 97: 39-47, 1991. PubMed: 1847347

Shipped freeze-dried
Shipping Information

Shipped:  Freeze dried E. coli containing the plasmid.

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