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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 0E-16 Vector: pRK2073 (plasmid) Promoters: Promoter none Construction: pRK2013, Tn7 Marker(s):tmpR Construct size (kb): 0.0 Features: marker(s): tmpR
promoter: none
replicon: RK2
terminator: none
enhancer: none
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Applications
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mobilization helper plasmid
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Comments
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The total size of the plasmid is greater than 48 kb. A helper plasmid which carries the conjugal transfer genes of RK2. This is a derivative of pRK2013 ( ATCC 37159) containing a Tn7 insert in the kanamycin resistance gene. |
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References
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Knauf VC, Nester EW. Wide host range cloning vectors: a cosmid clone bank of an Agrobacterium ti plasmid. Plasmid 8: 45-54, 1982. PubMed: 6292969
Figurski DH, Helinski DR. Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc. Natl. Acad. Sci. USA 76: 1648-1652, 1979. PubMed: 377280
Better M, Helinski DR. Isolation and characterization of the recA gene of rhizobium meliloti. J. Bacteriol. 155: 311-316, 1983. PubMed: 6305915
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Shipping Information
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Shipped: Freeze dried E. coli containing the plasmid
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