RK2 (ATCC® 37125™)

Applications: shuttle vector  /  Depositors: DR Helinski

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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Designations RK2
Depositors DR Helinski
Biosafety Level 1
Vector Information
Size (kb): 56.0000000000000000
Vector: RK2 (plasmid)
Construction: natural isolate
Marker(s):ampR,kanR,tetR
Construct size (kb): 56.0
Features: marker(s): ampR, kanR, tetR
replicon: RK2
Applications
shuttle vector
Comments
natural isolate from Pseudomonas sp.
contains relaxation complex site (rlx).
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Meyer R, et al. Molecular vehicle properties of the broad host range plasmid RK2. Science 190: 1226-1228, 1975. PubMed: 1060178

Ditta G, et al. Broad host range DNA cloning system for Gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proc. Natl. Acad. Sci. USA 77: 7347-7351, 1980. PubMed: 7012838

Shipped FD
Shipping Information Distributed: freeze-dried
Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Product Sheet
Product Sheet
Product Sheet