Shigella flexneri GFP (ATCC® 12022GFP)

Type Strain: no  /  Biosafety Level: 2

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Application Fluorescence detection
Fluorescence imaging
FACS
Cell infection assays
In vivo localization
Pathogen-host interactions assays
Type Strain no
Biosafety Level 2
Antigenic Properties Serotype 2b
Product Format frozen
Storage Conditions -80°C or colder
Preceptrol® no
Type Strain no
Antigenic Properties Serotype 2b
Antibiotic Resistance Ampicillin resistance gene (bla) encoded on a plasmid. Confers resistance to 100 µg/mL ampicillin
Comments This clone was derived from ATCC® 12022™ and contains a multicopy vector encoding the green fluorescent protein GFPmut3. This gene is expressed under the control of the lacZ promoter. This construct has been designed for Gram-negative bacteria fluorescence labeling.

Vector: pUCP18-MCSgfpmut3
Vector size: 5.492kb
Vector type: plasmid
Marker: bla (ampR)
Features:
GFPmut3: fluorescent maker under the control of PlacZ promoter
Origin of replication: ori from pRO1600
Unique restriction sites: BamHI, EcoRI, EcoRV, SmaI, XhoI.
Double restriction sites: HindIII, PstI, SalI, XbaI.
Medium Medium 2853: Nutrient Agar/Broth with 100 mcg/ml Ampicillin
Growth Conditions Temperature: 37°C
Atmosphere: Aerobic
Name of Depositor M. Barbier
Chain of Custody Modified by ATCC (Dr. M. Barbier) <-- ATCC <-- CDC
References

Barbier M and Mittar D. Novel fluorescent reporters for studying pathogen-host interactions. ASM general meeting 2014 Poster.

Choi KH, Schweizer HP. mini-Tn7 insertion in bacteria with single attTn7 sites: example Pseudomonas aeruginosa. Nat. Protoc. 1(1): 153-161, 2006. PubMed: 17406227

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Restrictions
Restrictions

This product's use is governed by the Limited Use License. For information on purchasing a license to use this product for research (for-profit entities) or commercial purposes (any entity) other than those permitted in the limited use label license, contact the Licensing Department, Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, California 92008. Phone (760) 603-7200 or outlicensing@lifetech.com