Embryonic stem cells are best supported in vitro by growth on mitotically arrested feeder cells. Mitotic inactivation, by treatment of mouse embryonic fibroblasts (MEFs) and other feeder cell cultures with Mitomycin C, prevents division of the fibroblasts while allowing the cells to survive and produce key proteins such as Leukemia Inhibitory Factor (LIF) that support the self renewal cycle of mouse embryonic stem cells.
The use of Mitomycin C requires the implementation of stringent safety measures during use, since the compound is toxic to both cultured cells and to humans.
Mitomycin C is an antineoplastic antibiotic that inhibits DNA synthesis by cross-linking DNA at 5' —CpG— 3' sequences. It produces oxygen radicals and is preferentially toxic to hypoxic cells. Care must be taken when preparing the Mitomycin C solution. The fibroblasts must be extensively washed following treatment to prevent toxic effects to embryonic stem (ES) cells, or other cell types that require the use of a feeder cell layer for propagation.
Mitomycin C is a highly toxic substance. Do not swallow. It is carcinogenic and a teratogen. In case of accident, or if you feel unwell, seek medical advice immediately. MSDS available upon request.
CAS Number: 50-07-7
Molecular Weight: 334.3
Molecular Formula: C15H18N4O5