pGRN145 plasmid in E. coli GC10 (ATCC® MBA-141™)

Depositors: Geron Corporation

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • License agreement required for commercial customer uses.
  • This material is distributed for research purposes only. A signed addendum to the ATCC Material Transfer Agreement must be sent to ATCC in advance of shipment.
Designations pGRN145 plasmid in E. coli GC10
Depositors Geron Corporation
Applications Expression vector [telomerase reverse transcriptase] (EST2; TCS1; TP2; TRT; hEST2; telomerase catalytic subunit)
Vector
Total size (kb): 14.002
Bacterial selection marker: cmlR

 

Features:
hTERT: 4225-7623
HygB: 9216-10238 (complementary)
CatR: 10822-11480
AmpS: 12941-13799 (complementary)
PAC: 368-964 (complementary)
F1 ori: 11501-11685 (complementary)
MPSV: 3643-4119 (promoter)
Ori: 12165-12747

 

Restriction digests of this clone gave the following sizes (in kb): HindIII/KpnI - 10.5, 3.5; EcoRI - 10.6, 3.4; XhoI - 12.0, 2.0.
Insert
Gene: Telomerase reverse transcriptase (TERT)
Source: Human
Insert size (kb): 3.398
GenBank accession: NM_003219
Media ATCC® Medium 2585: LB Medium (ATCC medium 1065) with 37 mcg/ml chloramphenicol
Biosafety Level 1
Shipping Information Frozen glycerol stock of E. coli GC10 containing the plasmid
Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • License agreement required for commercial customer uses.
  • This material is distributed for research purposes only. A signed addendum to the ATCC Material Transfer Agreement must be sent to ATCC in advance of shipment.
Restrictions

This material is subject to claims under U.S. Patent Nos. 6,261,836 and 6,337,200, other pending patent applications, and foreign counterparts thereof. It is required that either the Addendum for Commercial and For-Profit Organizations or the Addendum for Noncommercial and Academic Organizations, as appropriate, be signed and returned to ATCC before shipment.

Product Sheet
Product Sheet
Product Sheet
Attachments MSDS
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