Specialty Cell Culture Media

  • Stem Cell Dissociation Reagent (ATCC® ACS-3010)

    ATCC® Number: ACS-3010
    Quantity: 250 mg
    Storage: 2°C to 8°C

    For-Profit:   $92.00 Non-Profit:   $92.00

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    Product Format: 250 mg
    Comments:

    Stem Cell Dissociation Reagent is a neutral protease isolated from Bacillus polymyxa that promotes safe and efficient detachment of human embryonic stem cells (hESC) and human induced pluripotent stem cells (hiPSC) during subcultivation in cell culture. The gentle proteolytic action of the reagent does not damage the cell membrane and supports the growth of hiPSCs in an undifferentiated state. Serum does not inhibit the activity of the reagent. Stem Cell Dissociation Reagent is suitable for hESCs and hiPSCs cultured in feeder-free and feeder-dependent conditions.

  • Dermal Cell Basal Medium (ATCC® PCS-200-030)

    ATCC® Number: PCS-200-030
    Quantity: 485 ml
    Storage: 2 to 8°C, protect from light
    Applications: Dermal Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC Primary Cell Solutions™ system.

    This system is designed to support cells (e.g., keratinocytes, melanocytes) derived from normal human epidermis. Dermal Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. 

    To support the proliferation and plating efficiency of keratinocytes (e.g., Primary Epidermal Keratinocytes, Normal, Human Neonatal Foreskin, ATCC PCS-200-010), Dermal Cell Basal Medium must be supplemented with the Keratinocyte Growth Kit (ATCC PCS-200-040). The complete serum-free (although not animal-free) media is formulated to inhibit fibroblast growth, and the low calcium concentration (0.06 mM) slows differentiation.

    To support the proliferation and plating efficiency of melanocytes (e.g., Primary Epidermal Melanocytes, Normal, Human Neonatal, ATCC PCS-200-012 or Primary Epidermal Melanocytes, Normal, Human Adult, ATCC PCS-200-013), Dermal Cell Basal Medium must be supplemented with either the Melanocyte Growth Kit (ATCC PCS-200-041) or the Adult Melanocyte Growth Kit (ATCC PCS-200-042).

    Using Dermal Cell Basal Medium supplemented with the appropriate growth kit, the growth of keratinocytes and melanocytes are supported without the use of feeder layers, extracellular matrix proteins or other substrates.

    Optional media supplements:

    1. Gentamicin-Amphotericin B Solution (ATCC PCS-999-025)
    2. Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)
    3. Phenol Red (ATCC PCS-999-001)

     

    For-Profit:   $51.00 Non-Profit:   $51.00

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  • For-Profit:   $26.50 Non-Profit:   $26.50

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  • Stem Cell Freezing Media (ATCC® ACS-3020)

    ATCC® Number: ACS-3020
    Quantity: 1 x 20 mL
    Storage: 2°C to 8°C

    For-Profit:   $51.00 Non-Profit:   $51.00

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    Product Format: frozen 1 x 20 mL
    Comments:
    Stem Cell Freezing Media is tested for pH, appearance, and sterility. Recovery, morphology and differentiation of hESCs and hiPSCs are confirmed after cryopreservation and thawing.
  • Stem Cell Freezing Media (ATCC® ACS-3020-3)

    ATCC® Number: ACS-3020-3
    Quantity: 3 x 20 mL
    Storage: 2°C to 8°C

    For-Profit:   $128.00 Non-Profit:   $128.00

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    Product Format: frozen 3 x 20 mL
    Comments:
    Stem Cell Freezing Media is tested for pH, appearance, and sterility. Recovery, morphology and differentiation of hESCs and hiPSCs are confirmed after cryopreservation and thawing.
  • Pluripotent Stem Cell SFM XF/FF (ATCC® ACS-3002)

    ATCC® Number: ACS-3002
    Quantity: 500 mL
    Storage: Complete medium is stored at -20°C

    For-Profit:   $235.00 Non-Profit:   $235.00

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    Product Format: frozen 500 mL
    Comments:
    This ready to use, complete medium, is tested for pH, osmolality, endotoxins and sterility. Each lot of Pluripotent Stem Cell SFM XF/FF is tested functionally with human stem cells to ensure that pluripotency is maintained as determined by expression of pluripotent markers.
  • ROCK Inhibitor Y27632 (ATCC® ACS-3030)

    ATCC® Number: ACS-3030
    Quantity: 10 mg
    Storage: -20°C or colder

    For-Profit:   $199.00 Non-Profit:   $199.00

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    Product Format: dried 10 mg
    Comments:
    Purity is > 98% as confirmed by HPLC analysis. Structure and mass are confirmed by NMR and mass spectrometry.
    Reconstituted product can be aliquoted and stored at -20°C for up to six months. Protect from light.
  • CellMatrix Basement Membrane Gel (ATCC® ACS-3035)

    ATCC® Number: ACS-3035
    Quantity: 5 mL
    Storage: -80°C or colder

    For-Profit:   $209.00 Non-Profit:   $209.00

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    Product Format: frozen 5 mL
    Comments:
    Each lot of CellMatrix Gel is shown to promote the attachment of hESCs/hiPSCs and to maintain hESCs/hiPSCs in a pluripotent state as determined by the expression of Oct-4 and Nanog pluripotent markers.
    Store at -80°C in aliquots. Avoid repeated freezing and thawing.
  • For-Profit:   $152.00 Non-Profit:   $152.00

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  • Adipocyte Differentiation Toolkit for Adipose-Derived MSCs and Preadipocytes (ATCC® PCS-500-050)

    ATCC® Number: PCS-500-050
    Quantity: 1 kit
    Storage: -20°C (or -70°C for long-term storage)
    Applications:

    The Adipocyte Differentiation Toolkit for Adipose-derived MSCs and Preadipocytes contains medium and reagents designed to induce adipogenesis in actively proliferating Adipose-Derived Mesenchymal Stem Cells and Preadipocytes with high efficiency, and to support maturation of derived adipocytes during lipid accumulation. The Toolkit is composed of the following components:
    1. One vial containing 1 mL of AD Supplement for use in initiation of adipocyte differentiation
    2. One bottle containing 5 mL of ADM Supplement for use in maintaining adipocyte differentiation
    3. One bottle containing 100 mL of Adipocyte Basal Medium for use in both initiation and maintenance of adipocyte differentiation

    *The Adipocyte Differentiation Toolkit for Adipose-derived MSCs and Preadipocytes provides enough medium and reagents for differentiation of ~6.8 x 105 cells when plated at a recommended density of 18,000 viable cells/cm2 in 4 wells of a 6 well tissue culture format. 



    For-Profit:   $162.00 Non-Profit:   $162.00

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