Store medium at 2°C to 8°C in the dark when not in use.
General culture conditions to maintain undifferentiated,
pluripotent mouse ES cells are now well established, and
incremental improvements, such as the development of
Mouse ES Cell Basal Medium, are key to the ongoing
success of stem cell research.
Mouse ES Cell Basal Medium is a sterile liquid tissue culture
medium containing phenol red that has been optimized to
support the undifferentiated self-renewal cycle related to
mouse embryonic stem cell growth.
The medium contains a stable glutamine dipeptide, providing
a long shelf-life when handled and stored properly medium may be supplemented by addition of ES-Cell
Qualified Fetal Bovine Serum (FBS) or a serum replacement
designed for mouse ES cells.
This medium can support the growth of mouse ES cells on
either a mitotically inactivated layer of mouse embryonic
fibroblast (MEF) feeder cells, or in a feeder-free environment using gelatin-coated plates.