AlphaMEM with Nucleosides without L-Glutamine. (ATCC® 30-2604)

Product Format 500 mL
Storage Conditions Store medium at 2°C to 8°C in the dark when not in use.
Applications

AlphaMEM (Alpha Minimum Essential Medium) is an enriched modification of MEM that is widely used in mammalian cell culture. This formulation is made in Earle’s salts and contains ribonucleosides and deoxyribonucleosides.

Comments Supplement with 2 mM L-glutamine prior to use.
Biosafety Level N/A
Quality Control Specifications

Sterility tests are performed on each lot of medium using current USP methods. Each lot of medium is also tested for the ability to support the growth of several different cell lines using both sequential subcultures and plating efficiencies. pH, osmolality, and endotoxin tests are also performed as indicated below.

Test*

Specification

pH

7.0 to 7.4

Osmolality

275 to 305

mOsm/kg H2O

Sterility Testing

Pass

Endotoxin

≤ 0.25 EU/mL

Mycoplasma

Negative

Growth Promotion

Pass

*Please consult the Certificate of Analysis for lot-specific test results.


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