Classic Cell Culture Media

  • Dulbecco's Modified Eagle's Medium (DMEM) without Phenol Red (ATCC® 30-2601™)

    ATCC® Number: 30-2601 ™
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    Dulbecco's Modified Eagle's Medium (DMEM) is a basal medium widely used in mammalian cell culture. The medium is a modification of Basal Medium Eagle (BME) that contains a four-fold higher concentration of amino acids and vitamins. This formulation does not contain phenol red or L-glutamine.

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    Product Format: 500 mL
    Comments: Supplement with 4 mM L-glutamine prior to use.

  • DMEM: F-12 Medium (ATCC® 30-2006™)

    ATCC® Number: 30-2006 ™
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    DMEM: F-12 Medium contains 2.5 mM L-glutamine, 15 mM HEPES, 0.5 mM sodium pyruvate, and 1200 mg/L sodium bicarbonate.

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    Formulation: Formulation
    Product Format: 500 mL
    Comments:
    NOTE: This reduced level of sodium bicarbonate (NaHCO3, 1.2 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.

  • AlphaMEM with Nucleosides without L-Glutamine. (ATCC® 30-2604™)

    ATCC® Number: 30-2604 ™
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    AlphaMEM (Alpha Minimum Essential Medium) is an enriched modification of MEM that is widely used in mammalian cell culture. This formulation is made in Earle’s salts and contains ribonucleosides and deoxyribonucleosides.

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    Product Format: 500 mL
    Comments: Supplement with 2 mM L-glutamine prior to use.

  • AlphaMEM without Nucleosides without L-Glutamine (ATCC® 30-2605™)

    ATCC® Number: 30-2605 ™
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    AlphaMEM (Alpha Minimum Essential Medium) is an enriched modification of MEM that is widely used in mammalian cell culture. This formulation is made in Earle’s salts and does not contain nucleosides or L-glutamine.

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    Product Format: 500 mL
    Comments: Supplement with 2 mM L-glutamine prior to use.

  • RPMI-1640 Medium (ATCC® 30-2001™)

    ATCC® Number: 30-2001 ™
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications: RPMI-1640 medium modified to contain 2 mM L-glutamine, 10 mM HEPES, 1 mM sodium pyruvate, 4500 mg/L glucose, and 1500 mg/L sodium bicarbonate, for use in incubators using 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.

    For-Profit:   $20.00 Non-Profit:   $20.00

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    Formulation: Formulation
    Product Format: 500 mL
    Comments:
    NOTE: This reduced level of sodium bicarbonate (NaHCO3, 1.5 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.

  • MCDB 131 Medium without L-Glutamine (ATCC® 30-2603™)

    ATCC® Number: 30-2603 ™
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    MCDB 131 Medium provides a defined and nutritionally balanced environment for mammalian cell culture. The medium includes trace elements and higher levels of certain vitamins and amino acids compared to basal medium. MCDB 131 Medium was originally developed for human microvascular endothelial cell (HUVEC) culture, but supports the growth of other cell types, including hepatocytes, smooth muscle cells, and cardiac myocytes.

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    Product Format: 500 mL
    Comments: Supplement with glutamine prior to use. For vascular endothelial cells, 10 mM glutamine (final concentration) is recommended. For other cell lines, 2 mM glutamine (final concentration) is generally required; however, the optimal concentration is dependent on your cell line of interest.

  • Dulbecco's Modified Eagle's Medium (DMEM) (ATCC® 30-2002™)

    ATCC® Number: 30-2002 ™
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    Dulbecco's Modified Eagle's Medium (DMEM) modified to contain 4 mM L-glutamine, 4500 mg/L glucose, 1 mM sodium pyruvate, and 1500 mg/L sodium bicarbonate. 

    For-Profit:   $20.00 Non-Profit:   $20.00

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    Product Format: 500 mL
    Comments:
    NOTE: This reduced level of sodium bicarbonate (NaHCO3, 1.5 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.

  • McCoy's 5A Medium (ATCC® 30-2007™)

    ATCC® Number: 30-2007 ™
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    McCoy's 5A Medium is modified to contain 1.5 mM L-glutamine and 2200 mg/L sodium bicarbonate.

    For-Profit:   $18.00 Non-Profit:   $18.00

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    Formulation: Formulation
    Product Format: 500 mL
    Comments:

    NOTE: This reduced level of sodium bicarbonate (NaHCO3, 2.2 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.

  • Endothelial Cell Growth Kit-VEGF (ATCC® PCS-100-041™)

    ATCC® Number: PCS-100-041 ™

    For-Profit:   $79.00 Non-Profit:   $79.00

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    Product Format: frozen 1 kit

  • Mouse ES Cell Basal Medium (ATCC® SCRR-2011™)

    ATCC® Number: SCRR-2011 ™
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications: General culture conditions to maintain undifferentiated,  pluripotent mouse ES cells are now well established, and  incremental improvements, such as the development of  Mouse ES Cell Basal Medium, are key to the ongoing  success of stem cell research.

    Mouse ES Cell Basal Medium is a sterile liquid tissue culture  medium containing phenol red that has been optimized to  support the undifferentiated self-renewal cycle related to  mouse embryonic stem cell growth.

    The medium contains a stable glutamine dipeptide, providing  a long shelf-life when handled and stored properly medium may be supplemented by addition of ES-Cell  Qualified Fetal Bovine Serum (FBS) or a serum replacement  designed for mouse ES cells. This medium can support the growth of mouse ES cells on  either a mitotically inactivated layer of mouse embryonic  fibroblast (MEF) feeder cells, or in a feeder-free environment using gelatin-coated plates.

    For-Profit:   $25.00 Non-Profit:   $25.00

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    Product Format: 500 mL