S194/5.XXO.BU.1 (ATCC® TIB-20™) Organism: Mus musculus, mouse / Cell Type: B lymphocyte General Information Characteristics Culture Method Specifications History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Organism Mus musculus, mouse Cell Type B lymphocyte Product Format frozen Morphology lymphoblast Culture Properties suspension Biosafety Level 1 Disease plasmacytoma; myeloma Strain BALB/c Applications Resistant to 0.1 mM 5-bromo-2'-deoxyuridine.Derived from the S194/5.XXO-1 cell line (ATCC TIB-19).Tested and found negative for ectromelia virus (mousepox). Derivation Derived from the S194/5.XXO-1 cell line (ATCC TIB-19). Antigen Expression H-2d; PC.1 Genes Expressed immunoglobulin; monoclonal antibody; not secreted,H-2d; PC.1 Comments Resistant to 0.1 mM 5-bromo-2'-deoxyuridine.Derived from the S194/5.XXO-1 cell line (ATCC TIB-19).Tested and found negative for ectromelia virus (mousepox). Complete Growth Medium Dulbecco's modified Eagle's medium, 90%; horse serum, 10% Subculturing Medium Renewal: Every 2 to 3 daysCultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. Isotype IgA (not secreted); kappa light chain (not secreted) Name of Depositor R Hyman, P Ralph, S Sarkar References Hyman R, et al. Cell-specific antigens and immunoglobulin synthesis of murine myeloma cells and their variants. J. Natl. Cancer Inst. 48: 173-184, 1972. PubMed: 4119881 Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis MSDS References Hyman R, et al. Cell-specific antigens and immunoglobulin synthesis of murine myeloma cells and their variants. J. Natl. Cancer Inst. 48: 173-184, 1972. PubMed: 4119881