F3B6 (ATCC® HB-8785)

Organism: human (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

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Organism human (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The cells are resistant to 6-thioguanine.
This line is suitable for fusing to human B cells for the production of human monoclonal antibodies.
Derivation
This line is suitable for fusing to human B cells for the production of human monoclonal antibodies.
Comments
Fusion of human peripheral blood B cell with NS-1 myeloma cells.
The cells are resistant to 6-thioguanine.
This line is suitable for fusing to human B cells for the production of human monoclonal antibodies.
Complete Growth Medium HL-1 medium with 2 mM L-glutamine, 99%; fetal bovine serum, 1%. HL-1 medium can be obtained from Lonza (catalog number 77201).
Complete Growth Medium
Culture Medium: HL-1 medium, 99%; fetal bovine serum, 1%. Iscove's modified
Dulbecco's medium with 10% fetal bovine serum has been used as an alternative
to HL-1.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp4 cells/ml and maintain between 5 X 10 exp4 and 1 X 10 exp6 cells/ml.
Cryopreservation
HL-1 medium, 82.5%, human serum albumin, 10%; DMSO, 7.5%
Name of Depositor Cetus Corp.
U.S. Patent Number
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
References

Larrick JW, Raubitschek AA. Pseudomonas aeruginosa exotoxin A antibodies, their preparation and use. US Patent 4,677,070 dated Jun 30 1987

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Larrick JW, Raubitschek AA. Pseudomonas aeruginosa exotoxin A antibodies, their preparation and use. US Patent 4,677,070 dated Jun 30 1987