F3B6 (ATCC® HB-8785™) Organism: human (B cell); mouse (myeloma) / Cell Type: hybridoma: B lymphocyte General Information Characteristics Culture Method History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Organism human (B cell); mouse (myeloma) Cell Type hybridoma: B lymphocyte Product Format frozen Morphology lymphoblast Culture Properties suspension Biosafety Level 1 Applications The cells are resistant to 6-thioguanine.This line is suitable for fusing to human B cells for the production of human monoclonal antibodies. Derivation This line is suitable for fusing to human B cells for the production of human monoclonal antibodies. Comments Fusion of human peripheral blood B cell with NS-1 myeloma cells.The cells are resistant to 6-thioguanine.This line is suitable for fusing to human B cells for the production of human monoclonal antibodies. Complete Growth Medium HL-1 medium with 2 mM L-glutamine, 99%; fetal bovine serum, 1%. HL-1 medium can be obtained from Lonza (catalog number 77201). Subculturing Medium Renewal: Every 2 to 3 daysCultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp4 cells/ml and maintain between 5 X 10 exp4 and 1 X 10 exp6 cells/ml. Cryopreservation HL-1 medium, 82.5%, human serum albumin, 10%; DMSO, 7.5% Name of Depositor Cetus Corp. References Larrick JW, Raubitschek AA. Pseudomonas aeruginosa exotoxin A antibodies, their preparation and use. US Patent 4,677,070 dated Jun 30 1987 Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis MSDS References Larrick JW, Raubitschek AA. Pseudomonas aeruginosa exotoxin A antibodies, their preparation and use. US Patent 4,677,070 dated Jun 30 1987