MC/CAR (ATCC® CRL-8083)

Organism: Homo sapiens, human  /  Cell Type: B lymphocyte  /  Tissue: peripheral blood  /  Disease: plasmacytoma; myeloma

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Organism Homo sapiens, human
Tissue peripheral blood
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2
Disease plasmacytoma; myeloma
Age 81 years
Gender male
Ethnicity Caucasian
Applications
Can be used as a human fusion line.

Storage Conditions liquid nitrogen vapor phase
Karyotype normal male diploid
Derivation
Although derived from cells taken from a patient with a plasmacytoma, this line has been shown to be an EBV-transformed B lymphoblastoid cell line.
Clinical Data male
Caucasian
81 years
Antigen Expression
HLA A1, A33/31, B7, B18, DRw1, DRw2; CD11a +; CD19 +; CD20 + CD28 -; CD38 +
Receptor Expression
complement (C3b)
Genes Expressed
immunoglobulin
Cellular Products
immunoglobulin
Tumorigenic Yes
Effects
Yes, in nude mice inoculated subcutaneously with 107 cells
Comments
The cells produce, but do not secrete, immunoglobulin.

No surface immunoglobulin.

8-azaguanine mutants may be cloned out or mutagenized for HAT sensitive cultures.

Complete Growth Medium Iscove's modified Dulbecco's medium, 80%; fetal bovine serum, 20%
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 x 105 cells/mL and maintain between 5 x 105 and 1 x 106 cells/mL.
Medium Renewal: Two to three times weekly.
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,13
D16S539: 11,12
D5S818: 11,14
D7S820: 9,13
THO1: 6,9
TPOX: 8
vWA: 17,18
Isoenzymes
ADA, 1
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
PGM1, 1
PGM3, 1
Isotype IgG1 (cytoplasmic); kappa light chain
Name of Depositor Research Corporation
References

Ritts RE Jr.. Human nonsecretory plasmacytoid cell line. US Patent 4,434,230 dated Feb 28 1984

Pellat-Deceunynk C, et al. Human myeloma cell lines as a tool for studying the biology of multiple myeloma: a reappraisal 18 years after. Blood 86: 4001-4002, 1995. PubMed: 7579375

Drexler H, et al. False human hematopoietic cell lines: cross-contaminations and misinterpretations. Leukemia 13: 1601-1607, 1999. PubMed: 10516762

Ritts RE J, et al. Establishment and characterization of a human non-secretory plasmacytoid cell line and its hybridization with human B cells. Int. J. Cancer 31: 133-141, 1983. PubMed: 6600719

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Ritts RE Jr.. Human nonsecretory plasmacytoid cell line. US Patent 4,434,230 dated Feb 28 1984

Pellat-Deceunynk C, et al. Human myeloma cell lines as a tool for studying the biology of multiple myeloma: a reappraisal 18 years after. Blood 86: 4001-4002, 1995. PubMed: 7579375

Drexler H, et al. False human hematopoietic cell lines: cross-contaminations and misinterpretations. Leukemia 13: 1601-1607, 1999. PubMed: 10516762

Ritts RE J, et al. Establishment and characterization of a human non-secretory plasmacytoid cell line and its hybridization with human B cells. Int. J. Cancer 31: 133-141, 1983. PubMed: 6600719

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.