SKO-007 (ATCC® CRL-8033-1)

Organism: Homo sapiens, human  /  Cell Type: B lymphocyte  / 

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Organism Homo sapiens, human
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Derivation
This line was derived from the U266 myeloma cell line of Nilsson RefOlsson L, Kaplan HS. Human-human hybridomas producing monoclonal antibodies of predefined antigenic specificity. Proc. Natl. Acad. Sci. USA 77: 5429-5431, 1980. PubMed: 6159646 by selecting for resistance to 8-azaguanine; the cells cells are HAT sensitive.
Antigen Expression
HLA A2, A3, B7, Bw60, Bw6, Cw3
Genes Expressed
immunoglobulin; monoclonal antibody
Cellular Products
immunoglobulin; monoclonal antibody
Comments
This is a batch of SKO-007 (formerly available as ATCC CRL-8033) that was cured at the ATCC of a mycoplasma contamination.
The J3 clone of SKO-007 is available also (ATCC CRL-8033-2).
Complete Growth Medium RPMI 1640 medium with 0.02 mg/ml 6-thioguanine, 1 mM sodium pyruvate and 2 mM L-glutamine, 85%; fetal bovine serum, 15%
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL.
Medium Renewal: 2 to 3 times per week
Cryopreservation Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 12,13
D13S317: 12
D16S539: 10
D5S818: 11,12
D7S820: 11,12
THO1: 5,7
TPOX: 8
vWA: 17
Isotype IgE; lambda light chain
Name of Depositor H Kaplan, Stanford University Board of Trustees
References

Olsson L, Kaplan HS. Human-human hybridomas producing monoclonal antibodies of predefined antigenic specificity. Proc. Natl. Acad. Sci. USA 77: 5429-5431, 1980. PubMed: 6159646

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Olsson L, Kaplan HS. Human-human hybridomas producing monoclonal antibodies of predefined antigenic specificity. Proc. Natl. Acad. Sci. USA 77: 5429-5431, 1980. PubMed: 6159646