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SKO-007 (ATCC^® CRL-8033-1^™)

Organism: Homo sapiens, human / Cell Type: B lymphocyte

Organism	Homo sapiens, human
Cell Type	B lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1
Applications	This line was derived from the U266 myeloma cell line of Nilsson (Clin.

Derivation	This line was derived from the U266 myeloma cell line of Nilsson (Clin. Exp. Immunol. 7:477-489, 1970) by selecting for resistance to 8-azaguanine;the cells cells are HAT sensitive.
Antigen Expression	HLA A2, A3, B7, Bw60, Bw6, Cw3
Genes Expressed	immunoglobulin; monoclonal antibody,HLA A2, A3, B7, Bw60, Bw6, Cw3
Comments	This line was derived from the U266 myeloma cell line of Nilsson (Clin. Exp. Immunol. 7:477-489, 1970) by selecting for resistance to 8-azaguanine;the cells cells are HAT sensitive. This is a batch of SKO-007 (formerly available as ATCC CRL-8033) that was cured at the ATCC of a mycoplasma contamination. The J3 clone of SKO-007 is available also (ATCC CRL-8033-2).

Complete Growth Medium	RPMI 1640 medium with 0.02 mg/ml 6-thioguanine, 1 mM sodium pyruvate and 2 mM L-glutamine, 85%; fetal bovine serum, 15%
Subculturing	Medium Renewal: 2 to 3 times per week Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Culture Conditions	Temperature: 37.0°C

STR Profile	Amelogenin: X,Y CSF1PO: 12,13 D13S317: 12 D16S539: 10 D5S818: 11,12 D7S820: 11,12 THO1: 5,7 TPOX: 8 vWA: 17
Isotype	IgE; lambda light chain

Name of Depositor	H Kaplan, Stanford University Board of Trustees
References	Olsson L, Kaplan HS. Human-human hybridomas producing monoclonal antibodies of predefined antigenic specificity. Proc. Natl. Acad. Sci. USA 77: 5429-5431, 1980. PubMed: 6159646

Basic Documentation	Product Sheet Certificate of Analysis MSDS
References	Olsson L, Kaplan HS. Human-human hybridomas producing monoclonal antibodies of predefined antigenic specificity. Proc. Natl. Acad. Sci. USA 77: 5429-5431, 1980. PubMed: 6159646