GK-5 ATCC ® CRL-1834™ Homo sapiens

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism	Homo sapiens, human
Cell Type	B lymphoblast; Epstein-Barr virus (EBV) transforme
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	2 Cells Contain HERPESVIRUS
Applications	GK-5 is a human lymphoblastoid cell line derived from the GM1500-GTG-A12 EBV transformed cell line. The cells secrete very low levels (less than 10 ng/ml) of human kappa light chain. The line has been used successfully to prepare human hybridomas.

Derivation	GK-5 is a human lymphoblastoid cell line derived from the GM1500-GTG-A12 EBV transformed cell line.
Genes Expressed	immunoglobulin; monoclonal antibody; against human kappa light chain
Comments	GK-5 is a human lymphoblastoid cell line derived from the GM1500-GTG-A12 EBV transformed cell line. The cells secrete very low levels (less than 10 ng/ml) of human kappa light chain. The line has been used successfully to prepare human hybridomas.

Complete Growth Medium	The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: 0.05 mM 2-mercaptoethanol fetal bovine serum to a final concentration of 10%
Subculturing	Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Name of Depositor	JF Kearney
References	Kearney JF. Hybridomas and monoclonal antibodies In: Kearney JF. Fundamental immunology: New YorkRaven Press pp. 751-766, 1984 Satoh J, et al. Human monoclonal autoantibodies that react with multiple endocrine organs. N. Engl. J. Med. 309: 217-220, 1983. PubMed: 6866036

Name of Depositor

JF Kearney

References

Kearney JF. Hybridomas and monoclonal antibodies In: Kearney JF. Fundamental immunology: New YorkRaven Press pp. 751-766, 1984

Satoh J, et al. Human monoclonal autoantibodies that react with multiple endocrine organs. N. Engl. J. Med. 309: 217-220, 1983. PubMed: 6866036

Permits

These permits may be required for shipping this product:

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

Basic Documentation

References

Kearney JF. Hybridomas and monoclonal antibodies In: Kearney JF. Fundamental immunology: New YorkRaven Press pp. 751-766, 1984

Satoh J, et al. Human monoclonal autoantibodies that react with multiple endocrine organs. N. Engl. J. Med. 309: 217-220, 1983. PubMed: 6866036

GK-5 (ATCC® CRL-1834™)