YB2/0 [YB2/3HL.P2.G11.16Ag.20] (ATCC® CRL-1662™)

Organism: Rattus norvegicus, rat  /  Tissue: spleen  /  Cell Type: hybridoma: B lymphoblast

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These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism Rattus norvegicus, rat
Tissue
spleen
Cell Type hybridoma: B lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
Tested and found negative for ectromelia virus (mousepox).
The cells are resistant to 8-azaguanine, are HAT sensitive and can be used as a fusion partner for rat B cells to make rat - rat hybridomas.
The cells do not secrete either heavy or light immunoglobulin chains.
This line was produced by fusing Y3/Ag1.2.3 (ATCC CRL-1631) cells with spleen cells from an AO strain rat.
Derivation
This line was produced by fusing Y3/Ag1.2.3 (ATCC CRL-1631) cells with spleen cells from an AO strain rat.
Comments
The cells do not secrete either heavy or light immunoglobulin chains.
The cells are resistant to 8-azaguanine, are HAT sensitive and can be used as a fusion partner for rat B cells to make rat - rat hybridomas.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures are maintained by addition or replacement of fresh medium. Start new cultures at 1 X 10 exp5 viable cells/ml and maintain at or below 1 X 10 exp6 cells/ml.
Name of Depositor C Milstein
References

Kilmartin JV, et al. Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line. J. Cell Biol. 93: 576-582, 1982. PubMed: 6811596

. . Methods Enzymol. 73B: 1-46, 1981.

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
References

Kilmartin JV, et al. Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line. J. Cell Biol. 93: 576-582, 1982. PubMed: 6811596

. . Methods Enzymol. 73B: 1-46, 1981.