HFF-1 IRR (ATCC® SCRC-1041.1™)

Organism: Homo sapiens, human  /  Tissue: Skin, foreskin  /  Cell Type: Fibroblast

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism Homo sapiens, human
Tissue Skin, foreskin
Cell Type Fibroblast
Product Format frozen
Morphology Fibroblast
Culture Properties Adherent
Biosafety Level 1

[Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS Publication No. (CDC) 93-8395. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office (2007). The entire text is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.htm]

 

 

 

 

 
Age newborn
Gender Male
Applications
Irradiated cells for use as feeder layer. These cells are provided to be used as feeder cells to support the growth of stem cells in the undifferentiated state.
Storage Conditions liquid nitrogen vapor phase
Clinical Data
Male
Comments
Once the feeder cells have attached, the culture medium can be changed to accommodate the cells to be supported. It is recommended that the feeder cells be plated 24 hours before use at 5 to 6 X 106/T75 in order to obtain a 100% confluent monolayer for stem cells growth.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 15%

    • This medium is formulated for use with a 5% CO2 in air atmosphere. (Standard DMEM formulations contain 3.7 g/L sodium bicarbonate and a 10% CO2 in air atmosphere is then recommended).
    Culture Conditions
    Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37°C
    Name of Depositor ATCC
    Passage History
    These cells are provided to be used as feeder cells to support the growth of stem cells in the undifferentiated state. They have been irradiated with 6,000 rads and will not replicate. The cells will begin to deteriorate in 2 to 3 weeks after plating. Once the feeder cells have attached, the culture medium can be changed to accommodate the cells to be supported. It is recommended that the feeder cells be plated 24 hours before use at 5 to 6 X 10(6)/T75 in order to obtain a 100% confluent monolayer for stem cells growth. It is not recommended to use them past passage no. 50 (P50).
    Year of Origin 2003
    References

    Amit M, et al. Human feeder layers for human embryonic stem cells. Biol. Reprod. 68: 2150-2156, 2003. PubMed: 12606388

    Hovatta O, et al. A culture system using human foreskin fibroblasts as feeder cells allows production of human embryonic stem cells. Hum. Reprod. 18: 1404-1408, 2003. PubMed: 12832363

    Andrews P, et al. Human embryonic fibroblast feeder cells. International Patent Application WO 03/078611 A1

    Permits Notice: Necessary Permits

    These permits may be required for shipping this product:

    • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
    Basic Documentation
    Product Sheet
    Product Sheet
    Certificate of Analysis
    Certificate of Analysis
    MSDS
    MSDS
    References

    Amit M, et al. Human feeder layers for human embryonic stem cells. Biol. Reprod. 68: 2150-2156, 2003. PubMed: 12606388

    Hovatta O, et al. A culture system using human foreskin fibroblasts as feeder cells allows production of human embryonic stem cells. Hum. Reprod. 18: 1404-1408, 2003. PubMed: 12832363

    Andrews P, et al. Human embryonic fibroblast feeder cells. International Patent Application WO 03/078611 A1