CoolCell® LX Alcohol-Free Cryopreservation Container (ATCC® ACS-6000)

Applications

The CoolCell® LX container, in combination with a -80°C freezer or dry ice locker, will provide the freezing rate of -1°C per minute that is ideal for cryo-preservation of most cultured cell lines.  

Biosafety Level N/A
Basic Documentation
Other Documentation
FAQs
  1. CoolCell LX Temperature
    The CoolCell can be used in a -70℃ to -80℃ environment. This includes mechanical freezers at -70℃ to -80℃ and dry ice lockers (typically stable at -75℃). The CoolCell will freeze at -1℃ per minut...
    Date Updated: 3/27/2014
  2. CoolCell LX cleaning
    CoolCell is a cross-linked closed-cell dense polyethylene foam. This makes it ideal for cleaning with aqueous detergents, alcohol, bleach, and acid/base viricide (such as Virkon S) solutions. Rin...
    Date Updated: 3/27/2014
  3. CoolCell LX Freezer Temperature
    The CoolCell is specifically designed to deliver a controlled -1°C/minute cell freeze rate in a -80°C freezer because most mammalian cell protocols recommend this method of cryopreservati...
    Date Updated: 3/27/2014
  4. Transfer samples from CoolCell LX
    When removing the cryo-preserved (frozen) cells from the CoolCell it is imperative to transfer the cells as quickly as possible to liquid nitrogen for archival storage. Prior to LN2 storage, we r...
    Date Updated: 3/27/2014
  5. CoolCell LX time in freezer


    Date Updated: 3/27/2014

  6. Number of vials in CoolCell LX
    It is good practice to fill all 12 wells with the same size vials with equal fill volumes of freezing media. As the CoolCell is a passive device, changes in the payload will result in minor chang...
    Date Updated: 3/27/2014
  7. CoolCell LX recycle time
    While a closed CoolCell will protect samples below -50℃ for 30 minutes, an open, empty CoolCell can be recycled to room temperature in just minutes for the next freeze cycle. After emptying sampl...
    Date Updated: 3/27/2014
  8. CoolCell LX pre-cooling
    Pre-cooling the CoolCell is not necessary. Unlike room-temperature alcohol filled containers that will re-warm chilled samples up to 12℃ even when placed immediately in the freezer, a room-temper...
    Date Updated: 3/27/2014
  9. CoolCell LX can be pre-cooled
    You can pre-cool CoolCell if you want, but unlike alcohol filled containers, an explosion-proof refrigerator is not necessary since no alcohol is present. Also, in side by side tests between an a...
    Date Updated: 3/27/2014
  10. CoolCell LX reduces freeze lag
    The time it takes between placing samples into the freezer and the onset of fusion is called – freezing lag. Minimizing exposure time to Dimethyl sulfoxide (DMSO) cryoprotectant solution be...
    Date Updated: 3/27/2014
  11. CoolCell LX cryovial type
    Yes, CoolCell is designed to accept any standard 1ml or 2ml cryovial. It doesn't matter if they are internally or externally threaded.
    Date Updated: 3/27/2014
  12. CoolCell LX solid state ballast
    The solid state ballast (core) never needs to be replaced (unless it's lost).
    Date Updated: 3/27/2014
  13. CoolCell LX routine maintenance
    No. CoolCell is virtually maintenance free. Just ensure the units are completely dry prior to placing in the freezer. All wells of the CoolCell must be dry before inserting vials, to ensure easy ...
    Date Updated: 3/27/2014
  14. CoolCell LX use limit
    The CoolCell is designed for an unlimited number of duty cycles with no change in performance. The CoolCell is precision machined from high density polyethylene foam and both the housing and allo...
    Date Updated: 3/27/2014