Human herpesvirus 2 (ATCC® VR-540)

Classification: Herpesviridae, Simplexvirus  /  Product Format: frozen 1 mL per vial

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Classification Herpesviridae, Simplexvirus
Deposited As Herpes simplex virus 2
Agent Human herpesvirus 2
Strain MS
Common Name Herpes simplex 2
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Images VR-540 image
Comments
Deposited as Herpes simplex virus 2.
Effect on Host
CPE, cell rounding, clumping, and sloughing
Recommended Host
Vero (ATCC® CCL-81™)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere while rocking continuously. End adsorption by adding virus growth medium.
Incubation: 2-5 days
Effect on Host
CPE, cell rounding, clumping, and sloughing
Name of Depositor WR Dowdle, K Herrmann, Centers for Disease Control and Prevention
Special Collection NCRR Contract
Source Brain of a 50 year old female with multiple sclerosis, Iceland, 1961
References

Dowdle WR, et al. Association of antigenic type of herpesvirus hominis with site of viral recovery. J. Immunol. 99: 974-980, 1967. PubMed: 4295116

Gudnadottir M, et al. Virus isolated from the brain of a patient with multiple sclerosis. Exp. Neurol. 9: 85-95, 1964. PubMed: 14126126

Pauls FP, Dowdle WR. A serologic study of herpesvirus hominis strains by microneutralization tests. J. Immunol. 98: 941-947, 1967. PubMed: 4290495

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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